2012 Fiscal Year Final Research Report
Gene expression mechanism by LysR-type transcriptional regulator and basis for self-recognition
| Project/Area Number |
22580078
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Shizuoka University |
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Principal Investigator |
OGAWA Naoto 静岡大学, 農学部, 教授 (60354031)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 遺伝子発現 / LysRタイプ転写調節因子 / 転写 / プロモーター |
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| Research Abstract |
The LysR-type transcriptional regulators (LTTRs) comprise one of the largest families of bacterial transcriptional regulators, involved in expression of many genes of diverse roles such as amino acid biosynthesis, degradation of aromatic compounds, and virulence factor synthesis. This study revealed important amino acids for DNA binding and inducer recognition by LTTR in relation to three dimensional structure, and established methods for purifying protein and DNA for co-crystallization of LTTR and the regulated promoter, which together will lead to elucidation of mechanism of transcriptional activation by LTTRs.
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