Study on bacterial hypervirulation by genomic rearrangement

2012 Fiscal Year Final Research Report

• Project/Area Number: 22580087
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Yamaguchi University
• Principal Investigator: AZAKAMI Hiroyuki 山口大学, 農学部, 准教授 (40263850)
• Co-Investigator(Kenkyū-buntansha): NOIRI Yuichro 大阪大学, 歯学研究科, 准教授 (50218286) ; EBISU Shigeyuki 大阪大学, 歯学研究科 (50116000)
• Project Period (FY): 2010 – 2012
• Keywords: 歯周病原性細菌 / ゲノム再編 / Eikenella corrodens

Research Abstract

We found that the genomic recombination occurs in periodontopathogenic bacterium, Eikenella corrodens, through phage infection. Moreover, we found that the genomic recombination leads to hypervirulation including the increase of lectin activity, biofilm formation, hemolytic activity, growth rate, and induction of inflammatory cytokine. We suggested that this hypervirulation might occur frequently in E. corrodens.

Research Products

• [Journal Article] LuxS Affects Biofilm Maturation and Detachment of the Periodontopathogenic Bacterium Eikenella corrodens (2013)
  • Author(s): MM. Karim, T. Hisamoto, T. Matsunaga, Y. Asahi, Y. Noiri, S. Ebisu, A. Kato, H. Azakami
  • Journal Title: Journal of Bioscience and Bioengineering
  • DOI: DOI:10.1016/j.jbiosc.2013.03.013
  • Peer Reviewed
• [Journal Article] The periodontopathogenic bacterium Eikenella corrodens produces anautoinducer-2-inactivating enzyme (2013)
  • Author(s): MM. Karim, A. Nagao, FJ. Mansur, T. Matsunaga, Y. Akakabe, Y. Noiri, S. Ebisu, A. Kato, H. Azakami
  • Journal Title: Bioscience, Biotechnology, and Biochemistry
  • DOI: DOI:10.1271/bbb.130047
  • Peer Reviewed
• [Journal Article] Synergistic effects of antibiotics and an N-acyl homoserine lactone analog on Porphyromonas gingivalisbiofilms (2012)
  • Author(s): Y. Asahi, Y. Noiri, J. Igarashi, H. Suga, Azakami H, S. Ebisu
  • Journal Title: Journal of Applied Microbiology Volume: 112 Pages: 404-411
  • Peer Reviewed
• [Journal Article] Antibiofilm Effects of Azithromycin and Erythromycin on Porphyromonas gingivalis (2011)
  • Author(s): H. Maezono, Y. Noiri, Y. Asahi, M. Yamaguchi, R. Yamamoto, N. Izutani, Azakami H, S. Ebisu
  • Journal Title: Antimicrobial Agents and Chemotherapy Volume: 55 Pages: 5887-5892
  • Peer Reviewed
• [Journal Article] Genomic Recombination through Plasmid- encoded Recombinase enhances Hemolytic Activity and Adherence to Epithelial Cells in the Periodontopathogenic Bacterium Eikenella corrodens. Bioscience (2011)
  • Author(s): T. Matsunaga, A. Nakayuki, Y. Saito, A. Kato, Y. Noiri, S. Ebisu, H. Azakami
  • Journal Title: Biotechnology, and Biochemistry Volume: 75 Pages: 748-751
  • Peer Reviewed
• [Journal Article] The Inhibitory Effects of Catechins on Biofilm Formation by the Periodontopathogenic Bacterium, Eikenella corrodens (2010)
  • Author(s): T. Matsunaga, A. Nakahara, KM. Minnatul, Y. Noiri, S. Ebisu, A. Kato, H. Azakami
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 74 Pages: 2445-2450
  • Peer Reviewed
• [Presentation] Purification and characterization of autoinducer-2 inactivating enzyme in periodontopathogenic bacterium Eikenella corrodens (2013)
  • Author(s): Karim Minnatul
  • Organizer: 日本農芸化学会(東北大学
  • Place of Presentation: 東北大学、仙台市
  • Year and Date: 2013-03-26
• [Presentation] オートインデューサーの不活性化による歯周病関連細菌Eikenella corrodens の口腔内コミュニケーション戦略 (2013)
  • Author(s): 阿座上弘行
  • Organizer: 日本細菌学会 ワークショップ「細菌間および細菌宿主間の相互作用」、日本細菌学会
  • Place of Presentation: 幕張メッセ、千葉市
  • Year and Date: 2013-03-18
• [Presentation] Purification and characterization of autoinducer-2 inactivating enzyme in periodontopathogenic bacterium Eikenella corrodens (2012)
  • Author(s): Mansur Jasin
  • Organizer: 日本分子生物学会
  • Place of Presentation: マリンメッセ福岡、福岡市
  • Year and Date: 2012-12-12
• [Presentation] Purification and characterization of autoinducer-2 inactivating enzyme in periodontopathogenic bacterium Eikenella corrodens (2012)
  • Author(s): Mansur Jasin
  • Organizer: Brawijaya International Agriculture 2012
  • Place of Presentation: Brawijaya Univeristy, Malang, Indonesia
  • Year and Date: 2012-12-04
• [Presentation] Effect of Autoinducer-2 on biofilm formation of periodontopathogenic bacterium Eikenella corrodens (2012)
  • Author(s): Karim Minnatul
  • Organizer: Brawijaya International Agriculture 2012
  • Place of Presentation: Brawijaya Univeristy, Malang, Indonesia
  • Year and Date: 2012-12-04
• [Presentation] 歯周病原性細菌Eikenella corrodens はファージ感染により口腔内で高病原化する (2012)
  • Author(s): 倉重吉宏
  • Organizer: 日本生物工学会
  • Place of Presentation: 神戸市神戸国際会議場
  • Year and Date: 2012-10-24
• [Presentation] 歯周病原性細菌Eikenella corrodens の溶血因子の精製と解析 (2012)
  • Author(s): 山本美保子
  • Organizer: 日本生物工学会
  • Place of Presentation: 神戸国際会議場、神戸市
  • Year and Date: 2012-10-24
• [Presentation] 歯周病原性細菌Eikenella corrodens はゲノム再編により高病原化する (2012)
  • Author(s): 阿座上弘行
  • Organizer: 日本農芸化学会中四国支部例会
  • Place of Presentation: 愛媛大学、松山市
  • Year and Date: 2012-06-02
• [Presentation] 歯周病関連細菌Eikenella corrodens はゲノム再編により高病原化する (2012)
  • Author(s): 阿座上弘行
  • Organizer: 日本細菌学会
  • Place of Presentation: 長崎ブリックホール、長崎市
  • Year and Date: 2012-03-27
• [Presentation] 歯周病原性細菌Eikenella corrodens のオートインデューサー2分解酵素の解析 (2012)
  • Author(s): Karim Minnatul:
  • Organizer: 日本農芸化学会
  • Place of Presentation: 京都女子大学、京都市
  • Year and Date: 2012-03-25
• [Presentation] 歯周病原性細菌Eikenella corrodens の溶血因子の精製と解析 (2012)
  • Author(s): 山本美保子
  • Organizer: 日本農芸化学会
  • Place of Presentation: 京都女子大学、京都市
  • Year and Date: 2012-03-25
• [Presentation] 歯周病原性細菌Eikenella corrodens はファージ感染により口腔内で高病原化する (2012)
  • Author(s): 倉重吉宏
  • Organizer: 日本農芸化学会
  • Place of Presentation: 京都女子大学、京都市
  • Year and Date: 2012-03-25
• [Presentation] 歯周病原性細菌Eikenella corroden (2011)
  • Author(s): 山田和範
  • Organizer: のゲノム再編機構の解析日本生物工学会
  • Place of Presentation: 東京農工大学、小金井市
  • Year and Date: 2011-09-27
• [Presentation] 歯周病原性細菌Eikenella corrodens のオートインデューサー2の分解に関する研究 (2011)
  • Author(s): 長尾章子
  • Organizer: 日本生物工学会
  • Place of Presentation: 東京農工大学、小金井市
  • Year and Date: 2011-09-26
• [Presentation] Genomic Recombination through Plasmid-Encoded Recombinase Enhances Hemolytic Activity and Adherence to Epithelial Cells in the Periodontopathogenic Bacterium Eikenella corrodens (2011)
  • Author(s): 阿 座 上 弘 行
  • Organizer: IUMS2011
  • Place of Presentation: 札幌コンベンションセンター、札幌市
  • Year and Date: 2011-09-10
• [Presentation] 歯周病原性細菌Eikenella corrodens の菌体表層レクチンはポーリン様のタンパク質である (2011)
  • Author(s): 松永哲郎
  • Organizer: 日本農芸化学会(震災のため大会は中止
  • Year and Date: 2011-03-27
• [Presentation] Autoinducer 2 from periodontopathogenic bacterium, Eikenella corrodens (2011)
  • Author(s): Karim Minnatul
  • Organizer: 日本農芸化学会
  • Year and Date: 2011-03-26
• [Presentation] Effect of genomic recombination in periodontopathogenic bacterium on its lectin-dependent adhesion to epithelial cells (2010)
  • Author(s): 松 永 哲 郎
  • Organizer: Pachichem 2010 Conference
  • Place of Presentation: Hawaii Convension Center, Honolulu, USA
  • Year and Date: 2010-12-16
• [Presentation] Purification of cell-surface lectin and its role in pathogenicity of periodontopathogenic bacterium, Eikenella corrodens (2010)
  • Author(s): 松永哲郎
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸国際会議場、神戸市
  • Year and Date: 2010-12-10
• [Presentation] 歯周病原性細菌Eikenella corrodens (2010)
  • Author(s): 松永哲郎
  • Organizer: の菌体表層レクチンの精製と解析日本生物工学会
  • Place of Presentation: フェニックス・シーガイア・リゾート、宮崎市
  • Year and Date: 2010-10-28
• [Presentation] 歯周病原性細菌Eikenella corrodens (2010)
  • Author(s): 長尾章子
  • Organizer: のオートインデューサー分解酵素の精製と解析、日本生物工学会
  • Place of Presentation: フェニックス・シーガイア・リゾート、宮崎市
  • Year and Date: 2010-10-28
• [Remarks]
  • URL: http://web.cc.yamaguchi-u.ac.jp/~azakami/