Production of Rare Oligosaccharides Using Microbial Enzymes

2012 Fiscal Year Final Research Report

• Project/Area Number: 22580088
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Kagawa University
• Principal Investigator: TAKATA Goro 香川大学, 希少糖研究センター, 准教授 (50322722)
• Co-Investigator(Kenkyū-buntansha): MORIMOTO Kenji 香川大学, 希少糖研究センター, 准教授 (90363184) ; YOSHIHARA Akihide 香川大学, 希少糖研究センター, 助教 (40548765)
• Project Period (FY): 2010 – 2012
• Keywords: 微生物酵素 / 糖質科学

Research Abstract

This research objective prospected to produce rare disaccharide and oligosaccharides by biotechnological methods. First, the material, D-allose was produced from starch by ethanol method. Although we tried to produce D-allose 1-phosphate via D-allose 6-phosphate, the efficiency was quite low. Therefore, we tried to produce this from glucose 1-phosphate using glucoside 3-dehydrogenase newly isolated by ourselves. We also investigate the oxidation of cellobiose using this microbe. As a result, we succeeded to oxidize cellobiose to ketocellobiose. By following reduction of sodium borohydride, we also succeeded to produce objective alosyl-allose at higher efficiency.

Research Products

• [Journal Article] Gene Cloning and Characterization of L-Ribulose 3-epimerase from Mesorhizobium loti and Its Application to Rare Sugar Production. (2013)
  • Author(s): Uechi, K., Takata, G., Fukai, Y., Yoshihara, A., Morimoto, K.
  • Journal Title: Biosci Biotechnol Biochem Volume: 77(3) Pages: 511-5
• [Journal Article] Preparation ofD-gulose from disaccharide lactitol using microbial and chemical methods (2013)
  • Author(s): Morimoto, K., Shimonishi, T., Miyake, S., Takata, G., Izumori, K.
  • Journal Title: BiosciBiotechnol Biochem Volume: 77(2) Pages: 253-8
• [Journal Article] Cloning and characterization of the L-ribose isomerase gene from Cellulomonas parahominis MB426. (2013)
  • Author(s): Morimoto, K., Terami, Y., Maeda, Y., Yoshihara, A., Takata, G., Izumori, K.
  • Journal Title: J Biosci Bioeng
• [Journal Article] Characterization of Mesorhizobium loti L-Rhamnose Isomerase and Its Application to L-Talose Production (2011)
  • Author(s): Takata, G., Uechi, K., Taniguchi, E., Kanbara, Y., Yoshihara, A., Morimoto, K., Izumori, K
  • Journal Title: Biosci Biotechnol Biochem Volume: 75(5) Pages: 1006-1009
• [Journal Article] Enzymatic and Mutational Analyses of a Class II 3,5-Cyclic Nucleotide Phosphodiesterase, PdeE, from Myxococcus xanthus (2011)
  • Author(s): Kimura, Y., Yoshimi, M., Takata, G.
  • Journal Title: J Bacteriol Volume: 193(8) Pages: 2053-2057
• [Journal Article] Cloning and overexpression of the xylitol dehydrogenase gene from Bacillus pallidus and its application to L-Xylulose production (2010)
  • Author(s): Takata, G., Poonperm, W., Morimoto, K., Izumori, K.
  • Journal Title: Cloning and overexpression of the xylitol dehydrogenase gene from Bacillus pallidus and its application to L-Xylulose production Volume: 74 (9) Pages: 1807-1813