2012 Fiscal Year Final Research Report
Conversion of hydrolases into transglycosidases
| Project/Area Number |
22580099
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
MORI Haruhide 北海道大学, 大学院・農学研究院, 准教授 (80241363)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 酵素化学 |
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| Research Abstract |
A variety of oligosaccharides can be produced by carbohydrate hydrolases. In this work, amino acid residues responsible for high transglycosylation activities were identified through the comparison of transglycosidases with hydrolases. It was clarified that one transglycosidase had a catalytic domain similar to anomer-inverting hydrolases, which possess no transglycosidase activity.
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Research Products
(29 results)
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[Journal Article] A Novel Metabolic Pathway for Glucose Production Mediated by α -Glucosidase-catalyzed Conversion of 1,5-Anhydrofructose.2012
Author(s)
Kim YM, Saburi W, Yu S, Nakai H, Maneesan J, Kang MS, Chiba S, Kim D, Okuyama M, Mori H, and Kimura A.
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Journal Title
J. Biol. Chem
Volume: 287
Pages: 22441-2244
DOI
Peer Reviewed
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[Journal Article] Novel dextranase catalyzing cycloisomaltooligosaccharide formation and identification of catalytic amino acids and their functions using chemical rescue approach.2012
Author(s)
Kim YM, Kiso Y, Muraki T, Kang MS, Nakai H, Saburi W, Lang W, Kang HK, Okuyama M, Mori H, Suzuki R, Funane K, Suzuki N, Momma M, Fujimoto Z, Oguma T, Kobayashi M, Kim D, and Kimura A.
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Journal Title
J. Biol. Chem
Volume: 287(24)
Pages: 19927-19935
DOI
Peer Reviewed
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[Journal Article] Sect. F, Struct.2011
Author(s)
Suzuki N, Kim YM, Fujimoto Z, Momma M, Kang HK, Funane K, Okuyama M, Mori H, and Kimura A.
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Journal Title
Biol. Cryst. Commun.
Volume: 67(Pt12)
Pages: 1542-1544
DOI
Peer Reviewed
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