Identification of a novel binding protein for the (pro)renin receptor

2013 Fiscal Year Final Research Report

• Project/Area Number: 22580102
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Gifu University
• Principal Investigator: NAKAGAWA Tsutomu 岐阜大学, 応用生物科学部, 准教授 (10281049)
• Project Period (FY): 2010-04-01 – 2014-03-31
• Keywords: （プロ）レニン受容体 / レニン-アンジオテンシン系 / 細胞内局在 / 小胞体 / トランスロケーション / 結合タンパク質

Research Abstract

The (pro)renin receptor (PRR) is a single transmembrane receptor for the pressor enzyme renin and its inactive precursor prorenin. PRR induces the catalytic activation of prorenin along with intracellular signaling. In this study, the glutathione S transferase-fused extracellular domain of PRR expressed in mammalian cells was observed by immunofluorescence microscopy to be localized in the endoplasmic reticulum-like membranous structure, and recovered in the detergent phase in detergent-based two-phase separation experiments, suggesting retention inside the cell through stable membrane association. By co-immunoprecipitation of FALG-tagged PRR with its interacting proteins in human cultured cells and mass spectrometry, GRP78/BiP was identified as a novel binding protein for PRR.

Research Products

• [Journal Article] Intracellular retention of the extracellular domain of the (pro)renin receptor in mammalian cells (2014)
  • Author(s): Suzuki-Nakagawa, C., Nishimura, M., Noda, M., Iwata, H., Hattori, M. Ebihara, A., Suzuki, F. and Nakagawa, T.
  • Journal Title: Biosci. Biotechnol. Biochem. Volume: (in press)
  • Peer Reviewed
• [Journal Article] Participation of the extracellular domain in (pro)renin receptor dimerization. Biochem (2014)
  • Author(s): Suzuki-Nakagawa, C., Nishimura, M., Tsukamoto, T., Aoyama, S., Ebihara, A., Suzuki, F. and Nakagawa, T.
  • Journal Title: Biophys. Res. Commun. Volume: 444 Pages: 461-466
  • Peer Reviewed
• [Presentation] 新規(プロ)レニン受容体結合タンパク質の同定と解析 (2013)
  • Author(s): 戸松千裕、荒川和希、廣瀬ひとみ、中川千春、海老原章郎、鈴木文昭、中川寅
  • Organizer: 日本農芸化学会中部支部第168回例会
  • Place of Presentation: 名古屋大学シンポジオン
  • Year and Date: 2013-10-12
• [Presentation] Extracellular domain of the (pro)renin receptor participates in dimerization (2013)
  • Author(s): Suzuki-Nakagawa, C., Nishimura, M., Tsukamoto, T., Aoyama, S., Ebihara, A., Suzuki, F. and Nakagawa, T.
  • Organizer: American Heart Association High Blood Pressure Research 2013 Scientific Sessions(アメリカ心臓協会高血圧研究会議)
  • Place of Presentation: ニューオーリンズ・マリオット(アメリカ合衆国ニューオーリンズ)
  • Year and Date: 2013-09-12
• [Presentation] (プロ)レニン受容体の多量体化における細胞外ドメインの重要性 (2013)
  • Author(s): 中川寅、中川千春、西村美沙、海老原章郎、鈴木文昭
  • Organizer: 血圧とホルモン科学協会第5回(プロ)レニン受容体フォーラム
  • Place of Presentation: 新宿ワシントンホテル
  • Year and Date: 2013-05-18
• [Presentation] 細胞内(プロ)レニン受容体の分子性状と細胞内分布 (2012)
  • Author(s): 西村美沙、中川千春、塚本知子、青山奨、海老原章郎、鈴木文昭、中川寅
  • Organizer: 第76回日本生化学会中部支部例会
  • Place of Presentation: 自然科学研究機構岡崎コンファレンスセンター
  • Year and Date: 2012-05-26
• [Presentation] (プロ)レニン受容体細胞外領域の細胞内局在への関与 (2011)
  • Author(s): 中川千春、西村美沙、野田雅子、海老原章郎、鈴木文昭、中川寅
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2011-09-24
• [Remarks]
  • URL: http://www.abios.gifu-u.ac.jp/nakagawa/