Structural determination and functional elucidation of novel thiolic glycoconjugates which can form microtube structures

2012 Fiscal Year Final Research Report

• Project/Area Number: 22580117
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bioproduction chemistry/Bioorganic chemistry
• Research Institution: Yokohama National University
• Principal Investigator: TAKEDA Minoru 横浜国立大学, 大学院・工学研究院, 准教授 (40247507)
• Co-Investigator(Renkei-kenkyūsha): UEDA Kazuyoshi 横浜国立大学, 大学院・工学研究院, 教授 (40223458)
• Project Period (FY): 2010 – 2012
• Keywords: 天然物質化学

Research Abstract

Terminal elongation of microtubes (sheaths) which are extracellulary produced by filamentous ion-oxidizing bacteria, Sphaerotilus natans and Leptothrix cholodnii, was confirmed by fluorescent microscopy. The sheaths were revealedto be commonly assembled from thiolic glycoconjugates composed of amphoteric glycan coreand dipeptide side chain containing glycine and cysteine. Considering their uniquechemical structures, the sheath-forming thiolic glycoconjugates were proposed to becollectively termed thiopeptidoglucan.

Research Products

• [Journal Article] A spatial relationship between sheath el ongation and cell proliferation in Sphaerotilus natans (2012)
  • Author(s): Minoru Takeda, Takuto Umezu, Yuta Kawasak i, Shoichi Shimura,Keiko Kondo, Koizumi Jun-ichi
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 76 Pages: 2357-2359
  • DOI: DOI:0.1271/bbb.120616
  • Peer Reviewed
• [Journal Article] Conformational analysis of an ext racellular polysaccharide produced by Sphaerot ilus natans (2012)
  • Author(s): Keiko Kondo, Minoru Takeda, Tsukasa Mashi ma, Masato Katahira, Jun-ichi Koizumi, Kazu yoshi Ueda
  • Journal Title: Carbohydrate Research Volume: 360 Pages: 102-108
  • DOI: DOI:10.1016/j.carres.2012.07.023.
  • Peer Reviewed
• [Journal Article] Patterns of sheath elongation, cell proliferation, and manganese(II) oxidation in Leptothrix cholodnii (2012)
  • Author(s): Minoru Takeda, Yuta Kawasaki, Takuto Umezu, Shoichi Shimura,Makoto Hasegawa and Jun-ichi Koizumi
  • Journal Title: Archives of Microbiology Volume: 194 Pages: 667-673
  • DOI: DOI:10.1007/s00203-012-0801-6
  • Peer Reviewed
• [Journal Article] Study of a novel glycoconjugate,thiopeptidoglycan, and a novel polysaccharide lyase, thiopeptidoglycan lyase (2011)
  • Author(s): Kondo Kondo, Minoru Takeda, Wataru Ejima, Yuta Kawasaki, Takuto Umezu, Mina Yamada, Jun-ichi Koizumi, Tsukasa Mashima, Masato Katahira
  • Journal Title: International Journal of Biological Macromolecules Volume: 48 Pages: 256-262
  • DOI: DOI:10.1016/j.ijbiomac.2010.11.009
  • Peer Reviewed
• [Presentation] Fluorescent labeling of the sheaths formed by the Sphaerotilus-Leptothrix group of bacteria (2011)
  • Author(s): Yuta Kawasaki, Takuto Umezu, Makoto Hasegawa, Keiko Kondo, Manami Sumikawa, Minoru Takeda
  • Organizer: International Congress of Bacteriology and AppliedMicrobiology
  • Place of Presentation: 札幌
  • Year and Date: 20110900
• [Presentation] Leptothrix cholodniiの鞘の蛍光標識化による伸長部位の特定 (2011)
  • Author(s): 河崎雄太、梅津卓人、 武田 穣、小泉淳一
  • Organizer: 日本農芸化学会
  • Place of Presentation: 京都
  • Year and Date: 20110300
• [Presentation] Microtube-forming glycoconjugate of bacterial origin (2010)
  • Author(s): Mina Yamada, Keiko Kondo, Yuta Kawasaki,Takuto Umezu, Minoru Takeda, Jun-ichi Koizumi
  • Organizer: 2010International Symposium on Advanced Biological Engineering
  • Place of Presentation: 北京
  • Year and Date: 20100700