2012 Fiscal Year Final Research Report
Comprehensive analysis of the biosynthetic machinery and their regulatory system for secondary metabolites in Streptomyces
| Project/Area Number |
22580121
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
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| Research Institution | Hiroshima University |
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Principal Investigator |
ARAKAWA Kenji 広島大学, 大学院・先端物質科学研究科, 准教授 (80346527)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 放線菌 / ポリケチド / 生合成 / ピロロキノリンキノン / 制御遺伝子 / オートレギュレーター / 転写活性化因子 / ゲノムマイニング |
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| Research Abstract |
We carried out comprehensive analysis of the biosynthetic machinery and their regulatory system for lankacidin and lankamycin production in Streptomyces rochei 7434AN4. The disruptant of lkmE encoding type-II thioesterase accumulated 15-nor-LM derivatives, suggesting LkmE is responsible for hydrolysis of aberrant starter units for LM biosynthesis. We elucidated the structure of the signaling molecules SRBs that induce LC and LM production. Furthermore, the extensive analysis of regulatory genes revealed the positive regulation of LM biosynthesis by two SARP genes.
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