2012 Fiscal Year Final Research Report
Distribution of lignin precursors in tree cells
| Project/Area Number |
22580183
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Wood science
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| Research Institution | Kyoto University |
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Principal Investigator |
YOSHINAGA Arata 京都大学, 大学院・農学研究科, 助教 (60273489)
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| Co-Investigator(Kenkyū-buntansha) |
KAMITAKAHARA Hiroshi 京都大学, 大学院・農学研究科, 准教授 (10293911)
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| Project Period (FY) |
2010 – 2012
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| Keywords | リグニン / 前駆物質 / コニフェリン / スクロース / 木質化 / 細胞壁 / MALDI-TOF MS / イメージング |
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| Research Abstract |
Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) was applied to detect monolignol glucosides in differentiating xylems of several Japanese softwoods. MALI-IMS combined with osmium tetroxide vapor treatment enabled us to distinguish distribution of sucrose and coniferin with the same molecular weight. MALDI-IMS using transverse sections of normal wood in Japanese cypress (Chamaecyparis obtusa) showed that coniferin was mainly distributed in tracheids during secondary wall formation.
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