2012 Fiscal Year Final Research Report
Proteomic analysis of acute phase proteins of sea cucumber,Apostichopus japonicus
| Project/Area Number |
22580208
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | Kitasato University |
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Principal Investigator |
NAKAMURA Osamu 北里大学, 海洋生命科学部, 准教授 (00306648)
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| Co-Investigator(Kenkyū-buntansha) |
TSUTSUI Shigeyuki 北里大学, 海洋生命科学部, 講師 (20406911)
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| Project Period (FY) |
2010 – 2012
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| Keywords | マナマコ / 体腔球 / リポ多糖 / リポテイコ酸 / ペプチドグリカン / ザイモサン / プロテオーム解析 / C-type レクチン |
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| Research Abstract |
To investigate acute-phase response proteins in a sea cucumberApostichopus japonicus, the coelomic fluids containing coelomocytes were obtained fromseveral specimens and divided into tubes. Bacterila- or fungi-derived substances suchas LPS and zymosan were added to those coelomic fluids. After incubation for 48h, thesupaernatant of coelomic fluids were subjected to 2-D electrophoresis and compared withthat of controls which were received the buffer only. As a result, many spots that werenot seen in the control were detected in the stimulated fluids. We obtained N-terminalamino acid sequences of several protein spots, and found some unidentified sequences aswell as C-type lectins reported formerly.
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