• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2012 Fiscal Year Final Research Report

Novel cell signaling by different Gb3-binding marine lectins.

Research Project

  • PDF
Project/Area Number 22580226
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Fisheries chemistry
Research InstitutionYokohama City University

Principal Investigator

OZEKI Yasuhiro  横浜市立大学, 大学院・生命ナノシステム科学研究科, 教授 (70275022)

Co-Investigator(Kenkyū-buntansha) YASUMITSU Hidetaro   (10182346)
Project Period (FY) 2010 – 2012
Keywordsレクチン / MytiLec / 一次構造 / グロボトリオース / ABCトランスポーター / SUEL/ラムノース結合レクチンファミリー
Research Abstract

A17kDa α-galactoside-binding lectin,MytiLec purified from the Mediterranean mussel was found to have a novel triple-tandem repeat structure. It recognized globotriose (Gb3)and appeared strong cell static activity againstGb3 expressing lymphoma Raji cells. Another α-galactoside-binding lectin, SAL,a member of SUEL/rhamnose-binding lectin family isolated from catfish eggdid not influence the growth of Raji cells, though it down-regulated the expression levels of the multidrug-resistanttransporter gene. Combining these results, it became clear that even lectins having same oligosaccharide binding specificity, can transmit different signals to cells according to the variations of their multivalency and dissociation constants. This new finding will be usefulto study the cellular functions of these lectins to regulate cell proliferation.

  • Research Products

    (27 results)

All 2013 2012 2011 2010 Other

All Journal Article (16 results) Presentation (3 results) Book (2 results) Remarks (6 results)

  • [Journal Article] bubble mixing (FBM) culture of E. coli: a highly cost-effective middle scale-size culture system2013

    • Author(s)
      Yasumitsu H, Tajima H, Isobe M, Kutsna S, Kawsar SMA, Fujii Y, Kanaly RA, Ozeki Y, Yokota E
    • Journal Title

      Protein Pept. Lett

      Volume: 20 Pages: 213-217

    • DOI

      DOI: 10.2174/0929866511320020014

  • [Journal Article] Domain composition of rhamnose-binding lectin from shishamo smelt eggs and its a carbohydrate-binding profile.2013

    • Author(s)
      Hosono M, Sugawara S, Tatsuta T, Hikita TKominami J, Nakamura-Tsuruta S, Hirabayashi J, Kawsar SMA, Ozeki Y, Hakomori-S-I, Nitta K
    • Journal Title

      Fish Physiol Biochem

    • DOI

      DOI:10.1007/s10695-013-9814-6

  • [Journal Article] MRP1 expressed on Burkitt's lymphoma cells was depleted by catfish egg lectin through Gb3-glycosphingolipid and enhanced cytotoxic effect of drugs.2012

    • Author(s)
      Fujii Y, Sugawara S, Araki D, Kawano T, Tatsuta T, Takahashi K, Kawsar SMA, Matsumoto R, Kanaly RA, Yasumitsu H, Ozeki Y, Hosono M, Miyagi T, Hakomori S-I, Takayanagi Y, Nitta K
    • Journal Title

      Protein J

      Volume: 31 Pages: 15-26

    • DOI

      DOI:10.1007/s10930-011-9369-2

  • [Journal Article] Cytotoxic and glycan-binding properties of an 18 kDa lectin isolated from themarine sponge Halichondria okadai2012

    • Author(s)
      Matsumoto R, Fujii Y, Kawsar SMA, Kanaly RA, Yasumitsu H, Koide Y, Hasan I, Ogawa Y, Im CH, Sugawara S, Hosono M, Nitta K, Hamako J, Matsui T, Ozeki Y
    • Journal Title

      Toxins

      Volume: 4 Pages: 323-338

    • DOI

      DOI:10.3390/toxins4050323

  • [Journal Article] Identification and recombinant analysis of botrocetin-2, a snake venom cofactor for von Willebrand factor-induced platelet agglutination2012

    • Author(s)
      Yamamoto-Suzuki Y, Sakurai Y, Fujimura Y, Matsumoto M, Hamako J, Kokubo T, Kitagawa H, Kawsar SMA, Fujii Y, Ozeki Y, Matsushita F, Matsui T
    • Journal Title

      Biochemistry

      Volume: 51 Pages: 5329-5338

    • DOI

      DOI:10.1021/bi300442c

  • [Journal Article] A lectin from the mussel Mytilus galloprovincialishas a highly novel primary structure and induces glycan-mediated cytotoxicity of globotriaosylceramide-expressing lymphoma cells2012

    • Author(s)
      Fujii Y, Dohmae N, Kawsar SMA, Matsumoto R, Hasan I, Koide Y, Kanaly RA, Yasumitsu H, Ogawa Y, Sugawara S, Hosono M, Nitta K, Hamako J, Matsui T, Ozeki Y
    • Journal Title

      J. Biol. Chem

      Volume: 287 Pages: 44772-44783

    • DOI

      DOI:10.1074/jbc.M112.418012

  • [Journal Article] Cytotoxicity and glycanbinding profile of a D-galactose-binding lectin from the eggs of a Japanese sea hare (Aplysia kurodai)2011

    • Author(s)
      Kawsar SMA, Matsumoto R, Fujii Y, Matsuoka H, Masuda N, Chihiro I, Yasumitsu H, Kanaly RA, Sugawara S, Hosono M, Nitta K, Ishizaki N, Dogasaki C, Hamako J, Matsui T, Ozeki Y
    • Journal Title

      Protein J

      Volume: 30 Pages: 509-519

    • DOI

      DOI:10.1007/s10930-011-9356-7

  • [Journal Article] D-galactoside-binding lectin purified from coronate moon turban, Turbo(Lunella) coreensiswith a unique amino acid sequence and the ability to recognize lacto-series glycosphingolipids2011

    • Author(s)
      Fujii Y, Matsumoto R, Kawsar SMA, Yasumitsu H, Ishizuka N, Dogasaki C, Hosono M, Nitta K, Hamako J, Matsui T, Ozeki Y
    • Journal Title

      Comp Biochem. Physiol. B Mol. Biol

      Volume: 158 Pages: 30-37

    • DOI

      DOI:10.1016/j.cbpb.2010.09.002

  • [Journal Article] Antiproliferative effects of galectin-1 from Rana catesbeianaeggs on human leukemia cells and its binding proteins in human cells2011

    • Author(s)
      Yasumitsu H, Mochida K, Yasuda C, Isobe M, Kawsar SMA, Fujii Y, Matsumoto R, Kanaly RA, Ozeki Y
    • Journal Title

      In Vitro Cell Dev Biol Anim

      Volume: 47 (10) Pages: 728-734

    • DOI

      DOI:10.1007/s11626-011-9462-z

  • [Journal Article] Glycomics of a novel type-2 N-acetyllactosamine-specific lectin purified from the feather star (Oxycomantus japonicus) Pelmatozoa (class Crinoidea)2011

    • Author(s)
      Matsumoto R, Shibata FT, Kohtsuka H, Sekifuji M, Sugii N, Nakajima H, Kojima N, Fujii Y, Kawsar SMA, Yasumitsu H, Ozeki Y
    • Journal Title

      Comp. Biochem. Physiol. B Mol. Biol

      Volume: 158 Pages: 266-273

    • DOI

      DOI:10.1016/j.cbpb.2010.12.004

  • [Journal Article] Toxicity assessment of a -galactoside-binding lectin (galectin-1) from bullfrog Rana catesbeianaunfertilized eggs2011

    • Author(s)
      Kawsar SMA, Aftabuddin S, Mostafa G, Yasumitsu H, Ozeki Y
    • Journal Title

      Acta Pharma. Sci

      Volume: 53 Pages: 107-115

  • [Journal Article] CGP stain: An inexpensive, odorless, rapid, sensitive, and in principle in vitro methylation-free Coomassie Brilliant Blue stain2010

    • Author(s)
      Yasumitsu H,Ozeki Y, Kawsar SMA, Toda T, Kanaly RA
    • Journal Title

      Anal. Biochem

      Volume: 406 Pages: 86-88

    • DOI

      DOI:10.1016/j.ab.2010.06.035

  • [Journal Article] RAMA stain: A fast, sensitive and less protein modifying CBB R250 stain2010

    • Author(s)
      Yasumitsu H, Ozeki Y, Kawsar SMA, Fujii Y, Sakagami M, Matsuo Y, Toda T, Katsuno H
    • Journal Title

      Electrophoresis 31

      Volume: 31 Pages: 1913-1917

    • DOI

      DOI:10.1002/elps.200900524

  • [Journal Article] Production of Active MMP7 in E. coliand its application for metalloproteinase inhibitors screening2010

    • Author(s)
      Katsuno H, Shirakawa R, Miyazaki K, Ozeki Y, Yasumitsu H
    • Journal Title

      Protein Pept. Lett.

      Volume: 17 Pages: 568-572

    • DOI

      DOI:http://dx.doi.org/10.2174/092986610791112648

  • [Journal Article] Inhibitory effects of RCG1, a β-galactoside-binding lectin from Rana catesbeiana(American bullfrog) oocytes against human and phytopathogens2010

    • Author(s)
      Kawsar SMA, Mamun SMA, Rahman MS, Yasumitsu H, Ozeki Y
    • Journal Title

      Canadian J. Pure Appl. Sci

      Volume: 4 Pages: 1141-1149

  • [Journal Article] Benz[a]anthracene biotransformation and production of ring fission products by Sphingobium sp. strain KK22

    • Author(s)
      Kunihiro M, Ozeki Y, Nogi Y, Hamamura N, Kanaly RA.
    • Journal Title

      Appl Environ Microbiol

    • DOI

      DOI:10.1128/AEM.01129-13

  • [Presentation] Depletion of multidrug transporter protein on cancer cells caused by lectin-glycosphingolipid interaction enhanced the chemotheraputic effects2012

    • Author(s)
      Fujii Y, Sugawara, S., Nitta, K, Hosono M, Tatsuta T, Ogawa Y, Kawsar SMA, Matsumoto R, Koide Y, Hasan I, Hakomori S-I, Ozeki Y
    • Organizer
      第26回国際糖質学会
    • URL

      http://ics2012madrid.com/

    • Place of Presentation
      スペイン共和国マドリッド市
    • Year and Date
      20120722-27
  • [Presentation] Hyper-diversification glycan-recognizing patterns and cell regulative activities of galactophilic lectins from marine invertebrtates2011

    • Author(s)
      Matsumoto R, Shibata FT, Kawsar SMA, Fujii Y, Yasumitsu H, Ozeki Y.
    • Organizer
      24thInternational Lectin Conference
    • Place of Presentation
      豪州ブリスベン市
    • Year and Date
      20110727-29
  • [Presentation] Glycomics study on marine invertebrate lectins to find the cell regulative activity2010

    • Author(s)
      Matsumoto R, Kawsar SMA, Fujii Y, Hasan I, Ozeki Y
    • Organizer
      9th International Marine Biotechnology Conference(第9回国際マリンバイオテクノロジー学会)
    • Place of Presentation
      中国、青島市
    • Year and Date
      20101008-12
  • [Book] Marine Proteins and Peptides: Biological Activities and Applications2013

    • Author(s)
      Se-Kwon Kim
    • Total Pages
      167-184ISBN:978-1-1183-7506-8
    • Publisher
      Wiley-Blackwell (Thomson Oxford, UK)
  • [Book] Close Up実験法Series222 CGP染色:無臭・安価で迅速な高感度SDSゲル染色法実験医学30巻

    • Author(s)
      頁安光英太郎、戸田年総、大関泰裕
    • Total Pages
      111-117
  • [Remarks]

    • URL

      http://en.wikipedia.org/wiki/Galactose_binding_lectin_domain

  • [Remarks]

    • URL

      http://www.uniprot.org/uniprot/B3EWR1

  • [Remarks]

    • URL

      http://www.shimoda.tsukuba.ac.jp/~jambio/ronbun.html#ozeki24

  • [Remarks]

    • URL

      http://www.shimoda.tsukuba.ac.jp/~jambio/ronbun.html#ozeki201305

  • [Remarks]

    • URL

      http://jglobal.jst.go.jp/detail.php?JGLOBAL_ID=201202270059150450&q=cgp%E6%9F%93%E8%89%B2&t=0

  • [Remarks]

    • URL

      http://www.bioregen.co.jp/products/item/rbs02.html

URL: 

Published: 2014-08-29   Modified: 2014-09-16  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi