2012 Fiscal Year Final Research Report
Identification of IgY receptor contributing for IgY uptake into avian egg yolks
| Project/Area Number |
22580307
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Zootechnical science/Grassland science
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| Research Institution | Nagoya University |
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Principal Investigator |
MURAI Atushi 名古屋大学, 生命農学研究科, 准教授 (10313975)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 畜産物利用 / ニワトリ / ウズラ / 卵黄 / 抗体 / IgY / 受容体 |
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| Research Abstract |
The present study was conducted to identify a IgY receptor contributing IgY uptake into avian egg yolks. The uptakes of recombinant IgY mutants into egg yolks were determined when intravenously injected into birds. The results showed that substitution of tyrosine residue at 363 of IgY markedly reduced IgY uptake into egg yolks. Since critical hurdle of Y363A mutant transport into egg yolks existed in the innermost layer of avian ovarian follicles, the inner most layer was used for isolation and identification of the IgY receptor. Seven candidate proteins for the IgY receptor was isolated from plasma membrane fraction of the inner most layer of ovarian follicles, which included yolk-precursor protein, vitellogenin. Further studies are necessary to clarify the precise mechanism of IgY uptake mediated by vitellogenin.
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[Journal Article] Avian IgY is selectively incorporated into the egg yolks of oocytes by discriminating Fc amino acid residues located on the Cυ3/Cυ4 interface.2013
Author(s)
Murai, A., Murota, R., DOI:, K., Yoshida, T., Aoyama, H., Kobayashi, M. and Horio, F.
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Journal Title
Develop. Comp. Immunol.
Volume: 39
Pages: 378-387
Peer Reviewed
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