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2012 Fiscal Year Final Research Report

Create international standard SNP markers for chicken, and theiranalysis systems

Research Project

  • PDF
Project/Area Number 22580319
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied animal science
Research InstitutionHiroshima University

Principal Investigator

NISHIBORI Masahide  広島大学, 大学院・生物圏科学研究科, 准教授 (80237718)

Project Period (FY) 2010 – 2012
Keywordsニワトリ / SNP / マーカー / DigiTag2 / 国際スタンダード / 96 組 / 集団解析 / 個体識別
Research Abstract

Single nucleotide polymorphisms (SNPs) have been used to identify genes responsible for economic traits, including genetic diseases in domestic animals, and to examine genet ic diversity of populations. In this study, the aim is to c reate international standard SNP markers for chicken, and their analysis systems. I genotyped 239 chicken autosomal SNPs using DigiTag2 assay to understand the genetic structure of four species of Junglefowls, the Japanese native chicken breeds , and the relationship of these breeds. Phylogenetic analysis using the concatenated 74 autosomal SNP genotypes distinguished four Junglefowls, all chickens and formed clusters of chickens belonging to the respective breeds. Based on the allele frequency data, the estimated probability of identity (P) was calculated to be 2.70×10-20. Since the FAOSTAT database published in 2011 showed that the total number of chickens raised worldwide was approximately 1.99 × 1010, this SNP set would have the power to identify all chickens in the world. More importantly, this set would be particularly useful to monitor the genetic structure of populations for maintenance of the characteristics of the chicken breeds and junglefowls.

  • Research Products

    (4 results)

All 2012 2011

All Journal Article (4 results) (of which Peer Reviewed: 4 results)

  • [Journal Article] Genetic relationships between Japanese native and commercial breeds using 70 chicken autosomal SNP genotypes by the DigiTag2 assay2012

    • Author(s)
      Takeshi Shimogiri , Nao Nishida, Miyuki Kudo, Kousuke Niwa , Masahide Nishibori, Keiji Kinoshita, Shin Okamoto, Yoshizane Maeda, Katsushi Tokunaga, and Hiroshi Yasue
    • Journal Title

      Animal Genetics

      Volume: 43(1) Pages: 98-103

    • Peer Reviewed
  • [Journal Article] Phylogenetic Analysis of Native Chicken from Bangladesh and Neighboring Asian Countries Based on Complete Sequence of Mitochondrial DNA D-loop Region2012

    • Author(s)
      Mohammed A. Islam, and Masahide Nishibori
    • Journal Title

      Journal of Poultry Science

      Volume: 49(4) Pages: 237-243

    • Peer Reviewed
  • [Journal Article] Islam. Production and price of indigenous naked neck and full feathered chicken reared under rural scavenging system in Bangladesh2012

    • Author(s)
      Mohammad F. Ahmed, Masahide Nishibori,and Mohammed A
    • Journal Title

      Journal of Agricultural Extension and Rural Development

      Volume: 4(4) Pages: 92-97

    • Peer Reviewed
  • [Journal Article] Japanese Silkie fowls are widely distributed in the phylogenetic tree derived from mitochondrial complete D-loop nucleotide sequences2011

    • Author(s)
      Jahan Rowshan, Miyuki Kumagae, Masahide Nishibori, Hiroshi Yasue and Yasuhiko Wada
    • Journal Title

      Journal of Poultry Science

      Volume: 48(3) Pages: 176-180

    • Peer Reviewed

URL: 

Published: 2014-08-29  

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