2012 Fiscal Year Final Research Report
Generation of transgenic chicken for visualization of germ cells
| Project/Area Number |
22580325
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | National Agriculture and Food Research Organization |
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Principal Investigator |
TAGAMI Takahiro 独立行政法人農業・食品産業技術総合研究機構, 畜産草地研究所・家畜育種繁殖研 究領域, 主任研究員 (60355104)
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| Co-Investigator(Renkei-kenkyūsha) |
OGAWA Haruko 帯広畜産大学, 動物・食品衛生研究センター, 准教授 (10400079)
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| Research Collaborator |
OISHI Isao 独立行政法人産業総合技術研究所, 健康工学研究部門, 主任研究員 (50314472)
KAGAMI Hiroshi 信州大学, 農学部・食料生産科学科, 教授 (80308303)
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| Project Period (FY) |
2010 – 2012
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| Keywords | ニワトリ / 始原生殖細胞 / トランスジェニックニワトリ / Vasa ホモログタンパク質(CVH) / レンチウイルスベクター |
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| Research Abstract |
Visualization of chicken germ cells is useful to generate for transgenic chickens and critical to understanding the differentiation and proliferationof germ cells. As a first step, chicken vasa homologue (CVH) promoter sequence was searched. We tried to produce the transgenic chicken line in which the germline cells were visualized by CVH promoter-driven YFP or GFP expression. However, no germ cells in gonads of thetransgenic chickens expressed the fluorescence signal.
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Research Products
(13 results)
