Study on bovine leukemia virus integration site and genomic mutationsin cattle that develop leukemia.

2012 Fiscal Year Final Research Report

• Project/Area Number: 22580357
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied veterinary science
• Research Institution: Nihon University
• Principal Investigator: SENTSUI Hiroshi 日本大学, 生物資源科学部, 教授 (10355167)
• Co-Investigator(Renkei-kenkyūsha): MURAKAMI Kenji 独立行政法人農業・食品産業技術総合研究機構, 動物衛生研究所, 上席研究員 (10355141) ; SUZUKI Kazuhiko 東京農工大学, 農学部, 講師 (00366626)
• Project Period (FY): 2010 – 2012
• Keywords: 家畜衛生 / 遺伝子 / ウイルス / 癌 / 細胞 / 組織 / 獣医学 / 牛白血病

Research Abstract

The integration sites of bovine leukemia virus(BLV)in leukemiccells were analyzed using 90 tumor tissues from 48 0f leukemic cattle. Although the integration sites were not located in a particular chromosome, the BLV provirus wasintegrated into transcription units at a frequency of 43.6% and the transcriptional direction of the proviruswas in accordance with that of the integrated host genes in 62.5%.The integration sites were located in introns of the host gene, excluding only one site.BLV provir1.ts did not favor integration near transcription start sites and CpG islands.It is suggested that the integration site of the BLV provirus in leukemi ceells is relatedto the suppression of viral gene expression.

Research Products

• [Journal Article] Identification of bovine leukocyte antigen class II haplotypes associated with variations in bovine leukemia virus proviral load in Japanese Black cattle. (2013)
  • Author(s): Miyasaka T, Sentsui H, Aida Y.
  • Journal Title: Tissue Antigens Volume: 81 Pages: 72-82
  • Peer Reviewed
• [Journal Article] Identification and diversity of bovine major histocompatibility complexclass II hap1otypes in Japanese Black and Holstein cattle in Japan. (2012)
  • Author(s): Miyasaka T, Takeshima S-N, Sentsui H, Aida Y.
  • Journal Title: J. Dairy Sci. Volume: 95 Pages: 420-431
  • Peer Reviewed
• [Journal Article] Bovine leukemia Virus integration site selection in cattle that develop leukemia (2011)
  • Author(s): Murakami H, Yamada T, Suzuki M, Nakahara Y, Suzuki K, Sentsui H.
  • Journal Title: Virus Research. Volume: 156 Pages: 107-112
  • Peer Reviewed
• [Journal Article] The diversity of bovine MHC class II DRB3 and DQAl alleles in different herds of Japanese Black and Holstein cattle in Japan. (2011)
  • Author(s): Miyasaka T, Ishibashi K、 Sentsui H, Aida Y.
  • Journal Title: Gene Volume: 472 Pages: 42-49
  • Peer Reviewed
• [Journal Article] Analysis of Syk expression in bovine lymphoma and persistent lympho- cytosis induced by bovine leukemia virus. (2011)
  • Author(s): Murakami H, Kuroiwa T, Suzuki K, Miura Y, Sentsui H
  • Journal Title: J Vet Med Sci Volume: 73 Pages: 41-45
  • Peer Reviewed
• [Presentation] 牛白血病ウ (2013)
  • Author(s): 宮坂卓、小熊圭祐、泉封博.
  • Organizer: 第155回日本獣医学会学術集会
  • Place of Presentation: 東京大学教養学部
  • Year and Date: 2013-03-29
• [Presentation] 牛白血病ウイルスの宿主ゲノム内組込み位置と病態との関連. (2012)
  • Author(s): 宮坂卓、小熊圭祐、泉封博.
  • Organizer: 第60回目本ウイルス学会学術集会.
  • Place of Presentation: グランキューブ大阪
  • Year and Date: 2012-11-13
• [Presentation] 牛白血病ウイルス(BLV)プロウイルスロードとBoLAクラスII遺伝子多型相関性の解析 (2012)
  • Author(s): 宮坂卓、泉封博、間陽子.
  • Organizer: 第21回日本組織適合性学会大会.
  • Place of Presentation: 明治大学
  • Year and Date: 2012-09-16
• [Presentation] 新規ウシ主要組織適合抗原(MHC)クラスH遺伝子ハプロタイプの同定とその分布. (2012)
  • Author(s): 宮坂卓、竹嶋伸之輔、泉封博、間陽子
  • Organizer: 第21回日本組織適合性学会大会.
  • Place of Presentation: 明治大学
  • Year and Date: 2012-09-15
• [Presentation] 牛白血病発症牛及び持続性リンパ球増多症発症牛におけるLun発現量の解析 (2010)
  • Author(s): 村上裕信、福井大介、鈴木和彦、泉封博.
  • Organizer: 第150回 日本獣医学会学術集会
  • Place of Presentation: 帯広畜産大学
  • Year and Date: 2010-09-16