2012 Fiscal Year Final Research Report

Study of barley P23k to utilize β-glucan as a biomass resource

Research Project

Project/Area Number	22580380
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Boundary agriculture
Research Institution	Nagoya City University
Principal Investigator	KIDOU Shinichiro 名古屋市立大学, 大学院・システム自然科学研究科, 教授 (60271847)
Project Period (FY)	2010 – 2012
Keywords	バイオマス
Research Abstract	P23k is a barley protein that is highly expressed inβ-glucan-rich tissues. To elucidate the relationship between P23k andβ-glucan synthesis, a gain of function analysis of P23k were performed. The β-glucan content in P23k-expressing transgenic rice was increased slightly. In addition, β-amylase was isolated as a candidate of P23k interacting protein by GST pull-down assay. These results suggest that P23k is involved in the synthesis ofβ-glucan in barley.