Translational regulation by Argonaute protein, MIWI, and poly(A)-binding proteins

2012 Fiscal Year Final Research Report

• Project/Area Number: 22580384
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: University of Tsukuba
• Principal Investigator: KASHIWABARA Shin-ichi 筑波大学, 生命環境系, 准教授 (00254318)
• Project Period (FY): 2010 – 2012
• Keywords: 精子形成 / 翻訳制御 / MIWI / ポリ(A)鎖結合タンパク質

Research Abstract

Poly(A)-binding protein, which binds poly(A) tail of eukaryotic mRNAs, is a central player of mRNA metabolism, including mRNA stability, translation, and decay. We have previously shown that poly(A)-binding proteins, PABPC1 and PABPC2, interact with a testis-specific Argonaute protein, MIWI/PIWIL1, suggesting that MIWI is involved in translational regulation during spermatogenesis. Contrary to our expectation, MIWI had no significant effects on translation, when tethered to reporter mRNAs. Moreover, MIWI was barely detected in translationally inactive mRNP fractions. These results suggest that MIWI is not implicated in, at least, translational repression. We also elucidated the functionof PABPC2, a testis-specific PABPC, using knock-out mice.

Research Products

• [Journal Article] Two functional forms of ACRBP/sp32 are produced by pre-mRNA alternative splicing in the mouse (2013)
  • Author(s): Kanemori, Y., Ryu, J.-H., Sudo, M., Niida-Araida, Y., Kodaira, K., Takenaka, M., Kohno, N., Sugiura, S., Kashiwabara, S., and Baba, T.
  • Journal Title: Biology of Reproduction Volume: 88 Pages: 1-8
  • DOI: DOI:10.1095/biolreprod.112.107425
  • Peer Reviewed
• [Journal Article] Protein disulfide isomerase-P5, down-regulated in the final stage of boar epididymal sperm maturation, catalyzes disulfide formation to inhibit protein function in oxidative refolding of reduced denatured lysozyme (2010)
  • Author(s): Akama, K., Horikoshi, T., Sugiyama, A., Nakahata, S., Akitsu, A., Niwa, N., Intoh, A., Kakui, Y., Sugaya, M., Takei, K., Imaizumi, N., Sato, T., Matsumoto, R., Iwahashi, H., Kashiwabara, S., Baba, T., Nakamura, M., and Toda, T.
  • Journal Title: Biochimica et Biophysica Acta Volume: 1804 Pages: 1272-1284
  • DOI: DOI:10.1016/j.bbapap.2010.02.004
  • Peer Reviewed
• [Presentation] Analysis of mice lacking testis-specific poly(A) polymerase (2012)
  • Author(s): 山岡悠太郎、柏原真一、馬場 忠
  • Organizer: TPAP/PAPOLB第35回日本分子生物学会年会
  • Place of Presentation: マリンメッセ福岡(博多)
  • Year and Date: 2012-12-11
• [Presentation] ポリ(A)鎖関連因子の精子形成における役割 (2012)
  • Author(s): 柏原真一
  • Organizer: 特定領域研究「生殖系列の世代サイクルとエピゲノムネットワーク」第5回公開シンポジウム
  • Place of Presentation: 京都大学(京都)
  • Year and Date: 2012-11-22
• [Presentation] RNA結合タンパク質による精子形成制御 (2011)
  • Author(s): 柏原真一
  • Organizer: 特定領域研究「生殖系列の世代サイクルとエピゲノムネットワーク」第4回公開シンポジウム
  • Place of Presentation: 千里ライフサイエンスセンター(豊中)
  • Year and Date: 2011-11-18
• [Presentation] Testis-specific Argonaute protein MIWI mediates translational silencing in a GW182-independent manner (2011)
  • Author(s): 山岡悠太郎、石田和之、柏原真一、木村正紀、岩崎千恵子、田中 文、馬場 忠
  • Organizer: The 2nd World Congress on Reproductive Biology
  • Place of Presentation: CAIRNS CONVENTION CENTRE(オーストラリア)
  • Year and Date: 2011-10-11
• [Presentation] Translational repression by testis-specific Argonaute protein PIWIL1 (2010)
  • Author(s): 石田和之、○柏原真一、木村正紀、田中文、岩崎千恵子、馬場 忠
  • Organizer: Cold Spring Harbor Meeting (Germ cells)
  • Place of Presentation: Cold Spring HarborLaboratory (ニューヨーク)(6)
  • Year and Date: 2010-10-06
• [Presentation] miRISCにおけるポリA鎖結合タンパク質の機能解析 (2010)
  • Author(s): 岩崎千恵子、柏原真一、石田和之、田中 文、馬場 忠
  • Organizer: 第12回日本RNA学会年会
  • Place of Presentation: 一橋記念講堂(東京)
  • Year and Date: 2010-07-28
• [Remarks]
  • URL: http://www.acroman.org/