2012 Fiscal Year Final Research Report
Glycosyl conjugation of functional natural products for improvement of their intestinal absorption.
| Project/Area Number |
22590009
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemical pharmacy
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| Research Institution | Nagoya City University |
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Principal Investigator |
MIZUKAMI Hajime 名古屋市立大学, 大学院・薬学研究科, 教授 (30128219)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 二次代謝糖転移酵素 / 生物転換 / 配糖体 / 機能性化合物 / 消化管吸収 |
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| Research Abstract |
The present investigation was carried out to glycosylate various natural compounds with useful physiological activities by using recombinant glycosyltransferases, and to compare the intestinal absorption of the glycosides. A wide array of plant secondary product glucosyltransferases were cloned from various plant sources and their biochemical functions were characterized. Apocarotenoid glucosyltransferases from Gardenia jasminoides, iridoid-specific glucosyltranferases from Catharanthus roseus, Lonicera japonica and G. jasminoides, and a prenylfuranocoumarin glucosyltransferase from Glehnia littoralis were successfully isolated and functionally characterized, providing a working platform for synthetic biology of plant secondary glucosides. A series of quercetin glycosides were enzymatically synthesized and their intestinal absorption and anti-inflammatory activity were compared.
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[Journal Article] Anti-allergic effects of enzymatically modified isoquercitrin (α-oligoglucosyl quercetin 3-O-glucoside), quercetin 3-O-glucoside, α-oligoglucosyl rutin, and quercetin, when administered orally to mice.2013
Author(s)
Makino, T., Kanemaru, M., Okuyama, S., Shimizu, R., Tanaka, H., Mizukami, H
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Journal Title
J.Nat. Med.
Volume: (in press)
DOI
Peer Reviewed
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[Journal Article] UGT75L6 and UGT94E5 mediate sequential glucosylation of crocetin to crocin in Gardenia jasminoides.2012
Author(s)
Nagatoshi, M., Terasaka, K., Owaki, M., Sota, M., Inukai, T., Nagatsu, A., Mizukami, H.
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Journal Title
FEBS Lett
Volume: 586
Pages: 1055 -1061
Peer Reviewed
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