2012 Fiscal Year Final Research Report
Screening for antifungal agents from microbial origin using a silkwormmodel of Candida infection
| Project/Area Number |
22590013
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemical pharmacy
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| Research Institution | Kitasato University |
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Principal Investigator |
UCHIDA Ryuji 北里大学, 薬学部, 講師 (60280632)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 抗真菌剤 / 天然資源 / 天然活性物質 / カイコ / in vivo スクリーニング |
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| Research Abstract |
The in vivo-mimic assay system using silkworm larvae was performed as the primary screening tool to discover antibiotics against Candida albicans. Microbial culture broths were screened in this in vivo-mimic assay system, and a new antifungal compound, designated BF-0003C, was isolated from the culture broth of Penicillium sp. BF-0003. BF-0003 had potent anti-C. albicans activity by the agar diffusion method with an inhibition zone of 22 mm at 10 μg/6 mm disk. Furthermore, C. albicans-infected silkworms survived when BF-0003C was injected at 25 to 100 μg/larva.
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