2012 Fiscal Year Final Research Report
Studies on the function utilization of sialic acid-containing Oligosaccharides peptide
| Project/Area Number |
22590033
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Chemical pharmacy
|
|---|
| Research Institution | 公益財団法人野ロ研究所 (2012)
The Noguchi Institute (2010-2011) |
|---|
Principal Investigator |
SUGAWARA Shuichi 公益財団法人野ロ研究所, 研究部, 研究員 (70462265)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
OSUMI Kenji 公益財団法人野口研究所, 研究部, 研究員 (90203778)
|
|---|
| Project Period (FY) |
2010 – 2012
|
| Keywords | 生薬 / 天然物化学 |
|---|
| Research Abstract |
This study relates to a human influenza virus infection inhibition using the SGP and the establishment of new production method of SGP. This report provide mainly an effective, efficient and time-saving strategy for the large-scale production of a sialylglycopeptide (SGP) and , which is a naturally abundant N-glycan isolated from hen's egg yolk. Another method for SGP production has been reported by Seko and colleague. In their method SGP was isolated from egg yolks by treatment with phenol, gel filtration, and successive rounds of chromatography on anion- and cation-exchange columns. On the other hand, in our method, from egg Yolks or de-lipidated egg Yolk, it is carried out by a simple operation and desalted by washing with water is absorbed to the SGP on the ODS resin after elution with an organic solvent, and obtain a final product proceeds to freeze-drying step as it is. In our work is carried out under neutral conditions, 9-sugar SGP which have one NeuAc-Gal also was detected. It
… More
was possible to separate 9-sugar SGP in our present method. This technology and our patents were licenced to pharmaceutical company (Fushimi Pharamceutical Co., Ltd.) in January 2011, and SGP was commercially available from March 2011 already industrial production of SGP was started then. The SGP and related compounds supply system is now being put into place.
Since it has a sugar chain structure identical to the human influenza virus receptor, the results of this study has allowed large supply of research materials. And we filed a patent application to conduct research and a method for manufacturing the SGP various derivatives. In this regard, the present study was to contribute to biochemical deployment for obtaining knowledge about viral infection, and it is expected to gain new knowledge against influenza virus infection prevented. It was able to give a solution also to the challenge of ensuring sufficient tools for interaction studies with virus hemagglutinin (HA) and human sialic acid-containing oligosaccharides. Acceleration of related research is expected by providing research tools. This study, in the "academic" and "practical terms" that you are expected, shows larger contribution of research on influenza virus infection and the significance of virus interaction study. Less
|
