2012 Fiscal Year Final Research Report
Development of new sampling technique of body odors and skin emanations for diagnostic analysis
| Project/Area Number |
22590048
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Shujitsu University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
SAITO Keita 就実大学, 薬学部, 助教/講師 (30454854)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 固相マイクロ抽出(SPME)法 / 非侵襲的サンプリング / 生体ガス / 皮膚浸出液 / 不飽和アルデヒド / バイオマーカー / 体臭分析 / ストレス評価 |
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| Research Abstract |
Noninvasive sampling and sensitive analytical methods for thedetermination of the odors and emanations released from human body were developed byfiber solid-phase microextraction (SPME)/gas chromatography-mass spectrometry(GC-MS) or in-tube SPME/liquid chromatography-mass spectrometry (LC-MS). Body odorcomponents, such as volatile aldehydes and sulfur compounds, in skin emissions andsecretions were analyzed by fiber SPME/GC-MS. Using this method, we examined theeffect of smoking and the deodorant effect by interaction with 2-nonenal and antioxidantfoods. On the other hand, cortisol, testosterone and dehydroepiandrosterone in saliva wereanalyzed by automated on-line in-tube SPME/LC-MS, and used for the evaluation ofexperimental stress and fatigue. In addition, in-tube SPME/LC-MS method was used forthe analysis of 8-isoprostane and 8-OHdG as an oxidative stress biomarker in urinesamples, and applied to examine the effect of smoking.
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