• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2012 Fiscal Year Final Research Report

Visualization ofβ3 adrenoceptor signaling for development of ligand screening.

Research Project

  • PDF
Project/Area Number 22590069
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biological pharmacy
Research InstitutionIwate Medical University

Principal Investigator

FUJIMOTO Yasuyuki  岩手医科大学, 薬学部, 准教授 (60317724)

Project Period (FY) 2010 – 2012
Keywordsアドレナリン受容体 / 蛍光 / 脂肪滴
Research Abstract

β3 adrenergic receptor acts for transmission ofadrenergic signaling on the cell surface of adipocytes activating hydrolysis of neutral lipids stored in the cells. In this study, I tried visualization of signaling mediated by β3 adrenoceptor. For this purpose, β3 adrenergic receptor, βarrestin2 and perilipin were fused withfluorescent proteins and expressed in mammalian cells. Distributions of fluorescence of these proteins were observed with microscopes.

  • Research Products

    (6 results)

All 2012 2011 2010

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (4 results)

  • [Journal Article] Ohashi-Kobayashi A. and Maeda M. Transporter associated with antigen processing-like (ABCB9) stably expressed in Chinese hamster ovary-K1 cells is sorted to the microdomains of lysosomal membranes.2011

    • Author(s)
      Fujimoto Y., Kamakura A., Motohashi Y., Ohashi-Kobayashi A. and Maeda M
    • Journal Title

      Biol. Pharm. Bull.

      Volume: 34 (1) Pages: 36-40.

    • DOI

      DOI:10.1248/bpb.34.36

    • Peer Reviewed
  • [Journal Article] Convergent synthesis of fluorescence-labeled probes of Annonaceous acetogenins and visualization of their cell distribution.2010

    • Author(s)
      Kojima N., Morioka T., Urabe D., Yano M, Suga Y., Maezaki N., Ohashi-Kobayashi A., Fujimoto Y., Maeda M., Yamori T., Yoshimitsu T. and Tanaka T.
    • Journal Title

      Bioorg. Med. Chem.

      Volume: 18 (24) Pages: 8630-8641

    • DOI

      DOI:10.1016/j.bmc.2010.10.004

    • Peer Reviewed
  • [Presentation] Escherichia coliATP合成酵素変異型cサブユニットの抑圧変異の解析2012

    • Author(s)
      高橋翔太、荒木信、藤本康之、前田正知
    • Organizer
      第51回日本薬学会東北支部大会
    • Place of Presentation
      青森市
    • Year and Date
      2012-10-07
  • [Presentation] ABC 輸送体 TAPL (ABCB9) のlysosome 局在化におけるN 末端領域膜貫通ドメインの寄与2011

    • Author(s)
      藤本康之、前田正知
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都市
    • Year and Date
      2011-09-22
  • [Presentation] Lysosomal targeting of ABC transporter TAPL (Transporter Associated with Antigen Processing Like) 招待公演2011

    • Author(s)
      Maeda M. and Fujimoto Y.
    • Organizer
      BIT's 4th Annual Protein and Peptide Conference
    • Place of Presentation
      Beijing China
    • Year and Date
      2011-03-24
  • [Presentation] ABC 輸送体 TAPL(ABCB9)の lysosome 局在化における N末端領域の寄与2010

    • Author(s)
      藤本康之、前田正知
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会
    • Place of Presentation
      神戸市
    • Year and Date
      2010-12-08

URL: 

Published: 2014-08-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi