Modulation of proliferation and differentiation capacitiesby solute carrier transporters in neural progenitor cells

2012 Fiscal Year Final Research Report

• Project/Area Number: 22590081
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Kanazawa University
• Principal Investigator: NAKAMICHI Noritaka 金沢大学, 薬学系, 准教授 (10401895)
• Co-Investigator(Kenkyū-buntansha): KATO Yukio 金沢大学, 薬学系, 教授 (30251440)
• Project Period (FY): 2010 – 2012
• Keywords: 脳・神経 / 脳神経疾患 / 神経科学 / 薬理学 / 輸送担体

Research Abstract

To clarify the physiological role of solute carrier (SLC) transporters expressed in neural progenitor cells (NPCs), we examined effects of SLC transporters on proliferation and differentiation capacities in NPCs. As a result, it was suggested that carnitine/organic cation transporter OCTN1/SLC22A4 may be primary organic cation transporter in NPCs, and may suppress proliferation and promote neuronal differentiation of NPCs via uptake of the antioxidant ergothioneine.

Research Products

• [Journal Article] Role of organic cation/carnitine transporter 1 in uptake of phenformin and inhibitory effect on complex I respiration in mitochondria (2013)
  • Author(s): hitara, Y., Nakamichi, N., Norioka, M., Shima, h., Kato, Y., Horie, T
  • Journal Title: in uptake of phenformin and in mitochondria Volume: 132 Pages: 32-42
  • DOI: DOI:10.1093/toxsci/kfs330.
  • Peer Reviewed
• [Journal Article] TP binding cassette transporters in two distinct compartments of skin contribute to transdermal absorption of a typical substrate (2013)
  • Author(s): Hashimoto, N., Nakamichi, N., Uwafuji, S., Yoshida, K., Sugiura, T., Tsuji, A., Kato, Y.
  • Journal Title: J. Control. Release Volume: 165 Pages: 54-61
  • DOI: DOI:10.1016/j.jconrel.2012.10.011.
  • Peer Reviewed
• [Journal Article] Functional expression of carnitine/organic cation transporter OCTN1 in mouse brain neurons: Possible involvement in neuronal differentiation (2012)
  • Author(s): Nakamichi, N., Taguchi, T., Hosotani, H., Wakayama, T., Shimizu, T., Sugiura, T., Iseki, S., Kato, Y
  • Journal Title: Neurochem. Int Volume: 61 Pages: 1121-1132
  • DOI: DOI:10.1016/j.neuint.2012.08.004.
  • Peer Reviewed
• [Journal Article] Transferrin receptor-1 suppresses neurite outgrowth in neuroblastoma Neuro2A cells (2012)
  • Author(s): Nakamura, Y., Nakamichi, N., Takarada, T., Ogita, K., Yoneda, Y.
  • Journal Title: Neurochem. Int. Volume: 60 Pages: 448-457
  • DOI: DOI:10.1016/j.neuint.2011.08.018.
  • Peer Reviewed
• [Journal Article] DZK1 regulates breast cancer resistance protein in small intestine (2011)
  • Author(s): Shimizu, T., Sugiura, T., Wakayama, T.,Kijima, A., Nakamichi, N., Iseki, S.,Silver, D.L., Kato, Y
  • Journal Title: Drug Metab. Dispos Volume: 39 Pages: 2148-2154
  • DOI: DOI:10.1124/dmd.111.040295.
  • Peer Reviewed
• [Journal Article] Pharmacokinetics and hepatic uptake of eltrombopag, a novel platelet-increasing agent (2011)
  • Author(s): Takeuchi, K., Sugiura, T., Umeda, S.,Matsubara, K., Horikawa, M., Nakamichi, N., Silver, D.L., Ishiwata, N., Kato, Y
  • Journal Title: Drug Metab. Dispos Volume: 39 Pages: 1088-1096
  • DOI: DOI:10.1124/dmd.110.037960.
  • Peer Reviewed
• [Journal Article] Functional Expression of Carnitine/Organic Cation Transporter OCTN1/SLC22A4 in mouse small intestine and liver (2010)
  • Author(s): Sugiura, T., Kato, S., Shimizu, T.,Wakayama, T., Nakamichi, N., Kubo, Y.,Iwata, D., Suzuki, K., Soga, T., Asano, M., Iseki, S., Tamai, I., Tsuji, A., Kato, Y.
  • Journal Title: Drug Metab. Dispos Volume: 38 Pages: 1665-1672
  • DOI: DOI:10.1124/dmd.110.032763
  • Peer Reviewed
• [Journal Article] Preferential inhibition by anti-diarrheic 2-methoxy-4-methylphenol of Ca2+influx across acquired N-methyl-D-aspartate receptor channels composed of NR1/NR2B subunit assembly (2010)
  • Author(s): Nakamichi, N., Fukumori, R., Takarada, T., Kambe, Y., Yamamoto, T., Matsushima, N., Moriguchi, N., Yoneda, Y
  • Journal Title: J. Neurosci. Res Volume: 88 Pages: 2483-2493
  • DOI: DOI:10.1002/jnr.22399.
  • Peer Reviewed
• [Presentation] odulation of proliferation and differentiation activity by solute carrier OCTN1/SLC22A4 in neural progenitor cells (2013)
  • Author(s): Nakamichi, N., Ishimoto, T., Hosotani,H., Sugiura, T., Kato, Y
  • Organizer: 24th Biennial Meeting ISN-ASN
  • Place of Presentation: Cancun Convention Center(Mexico)
  • Year and Date: 2013-04-24
• [Presentation] 中枢神経系疾患の薬物治療における膜輸送体OCTN1/SLC22A4の役割 (2013)
  • Author(s): 中道 範隆, 杉浦 智子, 加藤 将夫
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2013-03-30
• [Presentation] 神経幹細胞における膜輸送体OCTN1の機能的発現と細胞増殖に及ぼす役割. (2013)
  • Author(s): 中道 範隆, 石本 尚大, 細谷 拓史, 杉浦智子, 加藤 将夫
  • Organizer: 第86回日本薬理学会年会
  • Place of Presentation: 福岡国際会議場(福岡県)
  • Year and Date: 2013-03-23
• [Presentation] Regulation of maturation by solute carrier OCTN1/SLC22A4 in mouse cortical neurons (2012)
  • Author(s): Nakamichi, N., Hosotani, H., Taguchi,T., Sugiura, T., Kato, Y
  • Organizer: 11th Biennial Meeting of APSN and 55th Meeting of JSN
  • Place of Presentation: Kobe International Conference Center(Hyogo)
  • Year and Date: 2012-10-02
• [Presentation] Suppression of proliferation by carnitine/organic cation transporter OCTN1 (SLC22A4) in neural progenitor cells (2012)
  • Author(s): Ishimoto, T., Nakamichi, N., Hosotani,H., Sugiura, T., Kato, Y
  • Organizer: 11th Biennial Meeting of APSN and 55th Meeting of JSN, Kobe International
  • Place of Presentation: Conference Center (Hyogo)
  • Year and Date: 2012-10-02
• [Presentation] OCTN1/SLC22A4が神経細胞の増殖・分化に及ぼす影響 (2012)
  • Author(s): 中道 範隆, 若山 友彦, 杉浦 智子, 井関尚一, 加藤 将夫
  • Organizer: 第7回トランスポーター研究会年会
  • Place of Presentation: 京都大学農学部(京都府)
  • Year and Date: 2012-06-10
• [Presentation] 膜輸送体OCTN1によるマウス神経芽腫細胞株Neuro2aの分化抑制 (2012)
  • Author(s): 中道 範隆, 細谷 拓史, 田口 貴之, 杉浦智子, 加藤 将夫
  • Organizer: 第85回日本薬理学会年会,国立京都国際会館
  • Place of Presentation: 京都府
  • Year and Date: 2012-03-16
• [Presentation] Involvement of OCTN1/SLC22A4 in uptake of ergothioneine and neurite outgrowth in murine neurons (2011)
  • Author(s): Hosotani, H., Nakamichi, N., Taguchi,T., Wakayama, T., Sugiura, T., Iseki, S.,Kato, Y
  • Organizer: 日本薬物動態学会第26回年会
  • Place of Presentation: 広島国際会議場(広島県)
  • Year and Date: 2011-11-18
• [Presentation] マウス神経細胞におけるカルニチン/有機カチオントランスポーターOCTN1の機能的発現 (2011)
  • Author(s): 中道 範隆, 田口 貴之, 細谷 拓史, 杉浦智子, 加藤 将夫
  • Organizer: 第54回日本神経化学会大会
  • Place of Presentation: 山代温泉 瑠璃光(石川県)
  • Year and Date: 2011-09-28
• [Presentation] マウス脳におけるカルニチン/有機カチオントランスポーターOCTN1(SLC22A4)の役割 (2010)
  • Author(s): 中道 範隆, 田口 貴之, 山田 寛哉, 杉浦智子, 加藤 将夫
  • Organizer: 第32回生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 富山国際会議場(富山県)
  • Year and Date: 2010-11-30
• [Remarks]
  • URL: http://www.p.kanazawa-u.ac.jp/~bunyaku/