Role of G protein-coupled receptor kinase 5 in myocardial infarction

2012 Fiscal Year Final Research Report

• Project/Area Number: 22590083
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Kyushu University
• Principal Investigator: NAKAYA Michio 九州大学, 薬学研究院, 准教授 (80464387)
• Project Period (FY): 2010 – 2012
• Keywords: 心臓 / G蛋白質共役型7回膜貫通型受容体キナーゼ / 心筋梗塞

Research Abstract

G protein-coupled receptor kinases (GRKs) were initially identified as molecules that phosphorylate G-protein coupled receptors, leading to their desensitization. We first found that GRK5 expression was significantly increased in the infarct area of left ventricle after myocardial infarction (MI). We next examined the mortality rate of WT and β-arrestin2 KO mice after MI. GRK5 KO mice were more prone to die than WT mice after MI.

Research Products

• [Journal Article] GRK6 deficiency in mice causes autoimmune disease due to impaired apoptotic cell clearance (2013)
  • Author(s): Michio Nakaya, Mitsuru Tajima, Hidetaka Kosako, Takeo Nakaya, Akiko Hashimoto, Kenji Watari, Hiroaki Nishihara, Mina Ohba, Shiori Komiya, Naoki Tani, Motohiro Nishida, Hisaaki Taniguchi, Yoji Sato, Mitsuru Matsumoto, Makoto Tsuda, Masahiko Kuroda, Kazuhid
  • Journal Title: Nat. Commun Volume: 4 Pages: 1532
  • DOI: DOI:10.1038/ncomms2540
  • Peer Reviewed
• [Journal Article] Attenuated desensitization of b-adrenergic receptor by water-soluble N-nitrosamines that induce S-nitrosylation without NO release (2013)
  • Author(s): Noriko Makita*, Yoji Kabasawa*, Yuko Otani, Firman, Junichiro Sato, Makiko Hashimoto, Michio Nakaya, Hiroaki Nishihara, Masaomi Nangaku, Hitoshi Kurose, Tomohiko Ohwada, Taroh Iiri
  • Journal Title: Circ. Res Volume: 112 Pages: 327-334
  • DOI: DOI:10.1161/CIRCRESAHA.112.277665
  • Peer Reviewed
• [Journal Article] Induction of cardiac fibrosis by b-blocker in G protein-independent but GRK5/b-arrestin2-dependent signaling pathways (2012)
  • Author(s): Michio Nakaya, Satsuki Chikura, Kenji Watari, Natsumi Mizuno, Koji Mochinaga, Supachoke Mangmool, Satoru Koyanagi, Shigehiro Ohdo, Yoji Sato, Tomomi Ide, Motohiro Nishida & Hitoshi Kurose
  • Journal Title: J. Biol. Chem Volume: 287 Pages: 35669-35677
  • DOI: DOI:10.1074/jbc.M112.357871
  • Peer Reviewed
• [Journal Article] Cilostazol suppresses angiotensin II-induced vasoconstriction viaprotein kinase A-mediated phosphorylation of the transient receptor potential canonical 6 channel (2011)
  • Author(s): Kinue Nishioka, Motohiro Nishida, Marina Ariyoshi, Zhong Jian, Shota Saiki, Mayumi Hirano, Michio Nakaya, Yoji Sato, Satomi Kita, Takahiro Iwamoto, Katsuya Hirano, Ryuji Inoue, & Hitoshi Kurose
  • Journal Title: Arterioscler. Thromb. Vasc. Biol Volume: 31 Pages: 2278-2286
  • DOI: DOI:10.1161/ATVBAHA.110.221010
  • Peer Reviewed
• [Journal Article] TRPC3-mediated Ca2+ influx contributes to Rac1-mediated production of reactive oxygen species in MLP-deficient mouse hearts (2011)
  • Author(s): Naoyuki Kitajima, Kenta Watanabe, Sachio Morimoto, Yoji Sato, Shigeki Kiyonaka, Masahiko Hoshijima, Yasuhiro Ikeda, Michio Nakaya, Tomomi Ide, Yasuo Mori, Hitoshi Kurose, & Motohiro Nishida
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 409 Pages: 108-113
  • DOI: DOI:10.1016/j.bbrc.2011.04.124
  • Peer Reviewed
• [Journal Article] Heterologous down-regulation of angiotensin type 1 receptors by purinergic P2Y2 receptor stimulation through S-nitrosylation of NF-kappaB (2011)
  • Author(s): Motohiro Nishida, Mariko Ogushi, ReikoSuda, Miyuki Toyotaka, Shota Saiki, Naoyuki Kitajima, , Michio Nakaya,Kyeong-Man Kim, Tomomi Ide, Yoji Sato,Kazuhide Inoue, & Hitoshi Kurose
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 108 Pages: 6662-6667
  • DOI: DOI:10.1073/pnas.1017640108
  • Peer Reviewed
• [Presentation] 心筋梗塞時におけるGRK5の役割解析 (2013)
  • Author(s): 仲矢道雄、黒瀬等
  • Organizer: 日本薬学会 第133年会
  • Place of Presentation: 横浜
  • Year and Date: 20130300
• [Presentation] アポトーシス細胞の貪食におけるGRK6の関与 (2012)
  • Author(s): 仲矢道雄、黒瀬等
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
  • Year and Date: 20121200
• [Presentation] 受容体シグナリングにおけるイメージング技術の応用 (2012)
  • Author(s): 仲矢道雄
  • Organizer: 日本薬学会 第132年会
  • Place of Presentation: 札幌
  • Year and Date: 20120300
• [Presentation] G タンパク質共役型受容体キナーゼ/βアレスチンを介した新規機能 (2012)
  • Author(s): 仲矢道雄、千蔵さつき、渡健治、西田基宏、黒瀬等
  • Organizer: 第85回日本薬理学会年会
  • Place of Presentation: 京都
  • Year and Date: 20120300
• [Presentation] βアドレナリン受容体遮断薬のGRK5/βアレスチン2を介した心臓の線維化 (2011)
  • Author(s): 仲矢道雄、西田基宏、黒瀬等
  • Organizer: 日本薬学会第131年会
  • Place of Presentation: 静岡
  • Year and Date: 20110300
• [Presentation] Induction of cardiac fibrosis by ss-blocker in G protein-independent but GRK5/ss-arrestin2-dependent signaling pathways (2011)
  • Author(s): 仲矢道雄、西田基宏、黒瀬等
  • Organizer: 第84回日本薬理学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20110300
• [Remarks]
  • URL: http://chudoku.phar.kyushu-u.ac.jp/index.html