2012 Fiscal Year Final Research Report
Study on some proteins controling cell death in oxidative stress-induced neuronal cell death.
| Project/Area Number |
22590091
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Suzuka University of Medical Science |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
INOUE Sumiko 鈴鹿医療科学大学, 薬学部, 准教授 (20378657)
HASHI Minako 金沢大学, 医学系研究科, 研究員(講師) (10272957)
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| Project Period (FY) |
2010 – 2012
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| Keywords | グルタミン酸 / 細胞死 / アポトーシス / プロテオミクス |
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| Research Abstract |
We have reported that glutamate exerts cytotoxic action through inhibition of cystine uptake, which leads to a marked decrease in cellular glutathione (GSH) levels exposing the cells to oxidative stresses leading to cell death. This cell death seemed to be apoptosis accompanying with 1-2Mbp giant DNA fragmentation led to internucleosomal DNA fragmentation. We expand on recent studies of intracellular proteins by examining the cell death regulating proteins or factors by using two-dimensional differential in-gel electrophoresis (2D-DIGE) in the intracellular peptides from cell extracts of C6 rat glioma cells. We identified a protein kinase C-δas one of protein kinases involved in the glutamate-induced cell death, and furthermore, identified 3 peptides derived from 10 proteins with LC-MS/MS in theglutamate-treated cells. Three peptides such as 78-kDa glucose-regulated protein (GRP78), annexin -A5and galectin-1 were increased in glutamate -treated glioma cells.
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[Presentation]2010
Author(s)
Higuchi, Y. Hashii, M., Tanii, H. and Kohriyama, Y.
Organizer
16th World Congress of Basic and Clinical Pharmacology
Place of Presentation
コペ ンハーゲン
Year and Date
2010-07-20
