2012 Fiscal Year Final Research Report

Elucidation of activation mechanism of human acetyl-CoA carboxylase

Research Project

Project/Area Number	22590113
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Drug development chemistry
Research Institution	The Institute of Physical and Chemical Research
Principal Investigator	KUNISHIMA Naoki 独立行政法人理化学研究所, タンパク質結晶構造解析研究グループ, グループ副ディレクター (70415149)
Co-Investigator(Renkei-kenkyūsha)	MATSUURA Yoshinori 独立行政法人理化学研究所, タンパク質結晶構造解析研究グループ, リサーチアソシエイト (50513462)
Project Period (FY)	2010 – 2012
Keywords	生活習慣病 / 脂肪酸生合成 / 結晶構造解析 / X線回折 / 酵素 / タンパク質 / 立体構造 / 薬物設計
Research Abstract	Toward the elucidation of the activation mechanism of acetyl-CoA carboxylase (ACC) that catalyzes the rate-limiting step of the fatty-acid biosynthesis, we tried the protein crystallography of the central domain of human ACC (hBCCP1, hBCCP2) and its activation enzyme biotin protein ligase (hBPL). The genes were synthesized for a large-scale expression using E.coli. These proteins were generally expressed as inclusion bodies that could not be solubilized by methods including the denaturation/refolding and the substitution of cysteine with alanine.

(1 results)