Analysis of the functions of the membrane protein basigin during spermatogenesis and in spermagozoa

2012 Fiscal Year Final Research Report

• Project/Area Number: 22590168
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Chiba University
• Principal Investigator: MAEKAWA Mamiko 千葉大学, 大学院・医学研究院, 助教 (20181571)
• Co-Investigator(Renkei-kenkyūsha): YAMATOYA Kenji 千葉大学, 大学院・医学研究院, 特任研究員 (80447309) ; ITO Chizuru 千葉大学, 大学院・医学研究院, 講師 (80347054) ; TOYAMA Yoshiro 千葉大学, 大学院・医学研究院, 講師 (70009637) ; TOSHIMORI Kiyotaka 千葉大学, 大学院・医学研究院, 教授 (20094097)
• Research Collaborator: CHEN Cheng 千葉大学, 大学院・医学研究院, 大学院生
• Project Period (FY): 2010 – 2012
• Keywords: 発生学 / 形態形成学

Research Abstract

To analyze the function of the basigin in the testis and sperm, we searched molecules which could bind to the basigin in the mouse testis and sperm. The results indicated that basigin did not co-localize with monocarboxylate transporter (MCT) 1, but with MCT2. Further, sperm-type basigin lacks a part of Ig-like C2 domain, compared to the testis-type basigin. It is also suggested that basigin in the sperm head is different from that in the sperm tail.

Research Products

• [Journal Article] Unique structures of organelles observed in primary spermatocytes after micro-injection of protein solutions such as immunoglobulin into the lumen of the seminiferous tubules in mice and rats (2012)
  • Author(s): Toyama Y, Chen C, Yamatoya K, Maekawa M, Ito C, Toshimori K
  • Journal Title: Andrologia Volume: (in press)
  • DOI: DOI:10.1111/and.12030
  • Peer Reviewed
• [Journal Article] Localization of RA175 (Cadm1), a cell adhesion molecule of the immunoglobulin superfamily, in the mouse testis and analysis of male infertility in the RA175-deficient mouse (2011)
  • Author(s): Maekawa M, Ito C, Toyama Y, Suzuki-Toyota F, Fujita E, Momoi T, Toshimori K
  • Journal Title: Andrologia Volume: 43(3) Pages: 180-188
  • DOI: DOI:10.1111/j.1439-0272.2010.01049.x
  • Peer Reviewed
• [Journal Article] Adhesion between the plasma membrane and the mitochondria in relation to migration of the cytoplasmic droplet during epididymal maturation in guinea pig spermatozoa (2010)
  • Author(s): Suzuki-Toyota F, Ito C, Maekawa M, Toyama Y, Toshimori K
  • Journal Title: Cel Tissue Res Volume: 341(3) Pages: 429-440
  • DOI: DOI:10.1007/s00441-010-1012-6
  • Peer Reviewed
• [Journal Article] Tetraspanin family protein CD9 in the mouse sperm: unique localization, appearance, behavior and fate during fertilization (2010)
  • Author(s): Ito C, Yamatoya K, Yoshida K, Maekawa M, Miyado K, Toshimori K
  • Journal Title: Cel Tissue Res Volume: 340(3) Pages: 583-594
  • DOI: DOI:10.1007/s00441-010-0967-7
  • Peer Reviewed
• [Journal Article] A model of the acrosome reaction progression via the acrosomal membrane-anchored protein equatorin (2010)
  • Author(s): Yoshida K, Ito C, Yamatoya K, Maekawa M, Toyama Y, Suzuki-Toyota F, Toshimori K
  • Journal Title: Reproduction Volume: 139(3) Pages: 533-544
  • DOI: DOI:10.1530/REP-09-0434
  • Peer Reviewed
• [Presentation] マウス精巣および精子における膜タンパク質ベイシジン分子の解析 (2013)
  • Author(s): 前川眞見子、大和屋健二、陳城、野崎正美、伊藤千鶴、年森清隆
  • Organizer: 第118回日本解剖学会総会・全国学術集会
  • Place of Presentation: サンポート高松・かがわ国際会議場(香川県)
  • Year and Date: 2013-03-30
• [Presentation] EQT-EGFP transgenic mouse を用いた time laps imaging と微細構造の対比による先体膜タンパク質 equatorin の挙動解析 (2013)
  • Author(s): 年森清隆、伊藤千鶴、大和屋健二、陳城、前川眞見子
  • Organizer: 第118回日本解剖学会総会・全国学術集会
  • Place of Presentation: サンポート高松・かがわ国際会議場(香川県)
  • Year and Date: 2013-03-30
• [Presentation] 抗equatorin 抗体はヒト先体マーカーとして有効である (2013)
  • Author(s): 伊藤千鶴、大和屋健二、陳城、前川眞見子、神村今日子、武藤透、年森清隆
  • Organizer: 第118回日本解剖学会総会・全国学術集会
  • Place of Presentation: サンポート高松・かがわ国際会議場(香川県)
  • Year and Date: 2013-03-30
• [Presentation] 精子先体赤道部は、先体反応の過程でIZUMO1 のflip-flop によるN 末端の露出の後、脆くなる (2013)
  • Author(s): 大和屋健二、伊藤千鶴、大澤光次郎、陳城、前川眞見子、岩間厚志、幡野雅彦、年森清隆
  • Organizer: 第118回日本解剖学会総会・全国学術集会
  • Place of Presentation: サンポート高松・かがわ国際会議場(香川県)
  • Year and Date: 2013-03-30
• [Presentation] Categorization of acrosome reaction and isolation of those sperm for biochemical analyses. International Symposium on the Mechanisms of Sexual Reproduction in Animal and Plants (2012)
  • Author(s): Yamatoya K, Ito C, Osawa M, Chen C, Maekawa M, Iwama A, Hatano M, Toshimori K
  • Organizer: Joint Meeting of the 2nd Allo-authentication Meeting and the 5th Egg-Coat Meeting(MCBEEC)
  • Place of Presentation: Hotel Nagoya Garden Palace. Nagoya (Japan)
  • Year and Date: 20121112-16
• [Presentation] マウス精巣および精子におけるベイシジン分子の解析 (2012)
  • Author(s): 前川眞見子、陳城、大和屋健二、伊藤千鶴、年森清隆
  • Organizer: 日本顕微鏡学会第68回学術講演会
  • Place of Presentation: つくば国際会議場(茨城県)
  • Year and Date: 2012-05-14
• [Presentation] 先体膜タンパク質Equatorin を指標したマウス先体形成の解析 (2012)
  • Author(s): 伊藤千鶴、大和屋健二、陳城、前川眞見子、年森清隆
  • Organizer: 日本顕微鏡学会第68回学術講演会
  • Place of Presentation: つくば国際会議場(茨城県)
  • Year and Date: 2012-05-14
• [Presentation] 初期の先体反応における精子の形態とタンパク質の解析 (2012)
  • Author(s): 大和屋健二、伊藤千鶴、陳城、前川眞見子、年森清隆
  • Organizer: 日本顕微鏡学会第68回学術講演会
  • Place of Presentation: つくば国際会議場(茨城県)
  • Year and Date: 2012-05-14
• [Presentation] マウス精巣および精子におけるモノカルボン酸トランスポーターの局在 (2012)
  • Author(s): 前川眞見子、大和屋健二、陳城、伊藤千鶴、外山芳郎、年森清隆
  • Organizer: 第117回日本解剖学会総会・全国学術集会
  • Place of Presentation: 山梨大学甲府キャンパス(山梨県)
  • Year and Date: 2012-03-28
• [Presentation] Fertilization analyzed by live imaging in the mouse (2012)
  • Author(s): Toshimori K, Ito C, Yamatoya K, Maekawa M, Toyama Y
  • Organizer: 第117回日本解剖学会総会・全国学術集会
  • Place of Presentation: 山梨大学甲府キャンパス(山梨県)
  • Year and Date: 2012-03-28
• [Presentation] Separatration of early stage acrosome reacted sperm and analysis of the proteins (2012)
  • Author(s): Yamatoya K, Ito C, Cheng C, Maekawa M, Toyama Y, Toshimori K
  • Organizer: 第117回日本解剖学会総会・全国学術集会
  • Place of Presentation: 山梨大学甲府キャンパス(山梨県)
  • Year and Date: 2012-03-28
• [Presentation] Expression of monocarboxylate transporters in the mouse testis and sperm, and their association with basigin (2011)
  • Author(s): Maekawa M, Yamatoya K, Chen C, Ando S, Ito C, Toyama Y, Toshimori K
  • Organizer: Proceeding of the 88th Annual Meeting of The Physiological Society of Japan and the 116th Annual Meeting of the Japanese Association of Anatomists
  • Place of Presentation: Yokohama (Japan)
  • Year and Date: 20110328-30
• [Presentation] Integration mechanism of a fertilization-related protein equatorin into the acrosomal membrane using transgenic mice (2011)
  • Author(s): Ito C, Yamatoya K, Yoshida K, Hatano M, Miyado K, Toyama Y, Maekawa M, Chen C, Toshimori K
  • Organizer: Proceeding of the 88th Annual Meeting of The Physiological Society of Japan and the 116th Annual Meeting of the Japanese Association of Anatomists
  • Place of Presentation: Yokohama (Japan)
  • Year and Date: 20110328-30
• [Presentation] It's a time to go back to "junctional specializations" in the testis (2011)
  • Author(s): Toyama Y, Ito C, Maekawa M, Yamatoya K, Toshimori K
  • Organizer: Proceeding of the 88th Annual Meeting of The Physiological Society of Japan and the 116th Annual Meeting of the Japanese Association of Anatomists
  • Place of Presentation: Yokohama (Japan)
  • Year and Date: 20110328-30
• [Presentation] Analysis of acrosomal proteins during acrosome reaction progression (2011)
  • Author(s): Yamatoya K, Ito C, Chen C, Maekawa M, Toyama Y, Toshimori K
  • Organizer: Proceeding of the 88th Annual Meeting of The Physiological Society of Japan and the 116th Annual Meeting of the Japanese Association of Anatomists
  • Place of Presentation: Yokohama (Japan)
  • Year and Date: 20110328-30
• [Presentation] Analysis of the basigin in the mouse sperm using three antibodies against different epitopes of the basigin molecule (2010)
  • Author(s): Maekawa M, Yamatoya K, Yoshida K, Ito C, Toyama Y, Suzuki-Toyota F, Toshimori K
  • Organizer: 11th International Symposium on Spermatology, Okinawa convention center
  • Place of Presentation: Okinawa (Japan)
  • Year and Date: 2010-06-25
• [Presentation] Tetraspanin family protein CD9 in the mouse sperm: unique localization , appearance, behavior and fate during fertilization (2010)
  • Author(s): Toshimori K, Ito C, Yamatoya K, Yoshida K, Maekawa M, Miyado K, Toyama Y
  • Organizer: 11th International Symposium on Spermatology, Okinawa convention center
  • Place of Presentation: Okinawa (Japan)
  • Year and Date: 2010-06-25
• [Presentation] Oocyte-activation ability correlates with presence of perinuclear theca substance MN13, whose organization is associated with acrosome formation (2010)
  • Author(s): Ito C, Yamatoya K, Yoshida K, Akutsu H, Yao R, Kyono K, Suzuki-Toyota F, Toyama Y, Maekawa M, Noda T, Toshimori K
  • Organizer: 11th International Symposium on Spermatology, Okinawa convention center
  • Place of Presentation: Okinawa (Japan)
  • Year and Date: 2010-06-25
• [Presentation] Further characterization of acrosomal protein Equatorin (2010)
  • Author(s): Yamatoya K, Yoshida K, Ito C, Maekawa M, Yanagida M, Araki Y, Miyado K, Toyama Y, Suzuki-Toyoda F, Toshimori K
  • Organizer: 11th International Symposium on Spermatology, Okinawa convention center
  • Place of Presentation: Okinawa (Japan)
  • Year and Date: 2010-06-25
• [Presentation] Strange organelles observed in primary spermatocytes after micro-injection of protein solutions into the seminiferous tubules (2010)
  • Author(s): Toyama Y, Suzuki-Toyota F, Maekawa M, Ito C, Yamatoya K, Yoshida K, Toshimori K
  • Organizer: 11th International Symposium on Spermatology, Okinawa convention center
  • Place of Presentation: Okinawa (Japan)
  • Year and Date: 2010-06-25
• [Remarks]
  • URL: http://www.m.chiba-u.ac.jp/class/devbiol/