2012 Fiscal Year Final Research Report
Spatiotemporal analysis of cyclic gene expression in the presomitic mesoderm using novel whole-embryo-live-imaging method
| Project/Area Number |
22590181
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Fujita Health University |
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Principal Investigator |
KONDOW Akiko 藤田保健衛生大学, 総合医科学研究所, 助教 (90396838)
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| Co-Investigator(Kenkyū-buntansha) |
OHNUMA Kiyoshi 長岡技術科学大学, 産学融合トップランナー養成センター, 准教授 (50396834)
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| Co-Investigator(Renkei-kenkyūsha) |
KOBAYASHI Tetsuya 東京大学, 生産技術研究所, 准教授 (90513359)
NONAKA Shigenori 基礎生物学研究所, 准教授 (90435529)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 発生学・形態形成学 / 体節形成 |
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| Research Abstract |
To analyze spatiotemporal aspects of the somitogenesis more in detail, we developed anovel experimental system describing spatiotemporal change of gene expression in thepresomitic mesoderm (PSM) with single-cell resolution in zebrafish embryo using noveldigital scanned laser light-sheet fluorescence microscopy. We successfully obtained 3Dtime-lapse images of zebrafish embryos including PSM at single-cell resolution. Theimages were recorded at 3 min intervals for more than 10 hours starting before the onsetof somite segmentation. To generate her1 reporter transgenic zebrafish,we in jected her1 reporter plasmid into zebrafish embryos.
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