2012 Fiscal Year Final Research Report
Investigation of the role of ABCF2, a regulator of cell volume, in induction of apoptosis.
| Project/Area Number |
22590215
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General physiology
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| Research Institution | Suzuka University of Medical Science |
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Principal Investigator |
赤塚 結子 鈴鹿医療科学大学, 薬学部, 准教授 (90321611)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 分子 / 細胞生理学 |
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| Research Abstract |
I found that the COOH-terminal region (amino acid 591- 616) of ABCF2 is necessary for its accumulation in the 100,000×g pellet after hypotonic stimulation.
On the other hand, I found that the content of ACTN4 accumulated in the 100,000× g pellet significantly increased, while the content of ABCF2 did not change, after the application of H_2O_2 or staurosporine. My results showed that this region was not necessary to regulate the behavior of ABCF2 after the application of H_2O_2 or staurosporine. The single-cell size measurements also revealed that AVD was not seenin cells expressing ABCF2 deletion mutant lacking this region.
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