2012 Fiscal Year Final Research Report

Effects of Neuropeptide W, and B on autonomic nervous system

Research Project

Project/Area Number	22590236
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	General pharmacology
Research Institution	University of Tsukuba
Principal Investigator	IRUKAYAMA Yoko 筑波大学, 分子行動科学研究コア, 研究員 (90312834)
Co-Investigator(Kenkyū-buntansha)	MURAKOSHI Nobuyuki 筑波大学, 医学医療系, 講師 (80447218) SAKURAI Takeshi 金沢大学, 医学系, 教授 (60251055)
Project Period (FY)	2010 – 2012
Keywords	生理活性 / ストレス / 循環器 / 高血圧
Research Abstract	In this study, we analyzed of the neuropeptide W (NPW) its receptor(NPBWR1), in autonomic functional regulation under stress, using NPB/WR1 deficient (Npbwr-/-) mice. The urinary catecholamines amount of Npbwr-/- mice was increased. To elucidate the role of NPW-NPBWR1 on acute stress, we created a stress model after having contact with the intruder, C57/BL6J mice for 30 minutes. We monitored heart rate, activity, and body temperature using a telemetry system. In Npbwr1-/- mice, recovery to the steady state of the heart rate after contact with the intruder was very slow compared with wild type mice. In Npbwr-/- mice, cardiac hypertrophy was increased in comparison with wild-type mice. After administration of angiotensin II to Npbwr-/- mice, the cardiac hypertrophy deteriorated significantly as compared with wild-type mice.