2012 Fiscal Year Final Research Report
Significances of modifying TNNI3 function by overexpression of MAP kinase TNNI3K
| Project/Area Number |
22590244
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General pharmacology
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| Research Institution | Kumamoto University |
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Principal Investigator |
YORINAKA Hoichi (LAI Zhong-fang) 熊本大学, 生命科学研究部, 助教 (90244110)
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| Co-Investigator(Kenkyū-buntansha) |
YORINAKA Gyokuchin 熊本大学, 生命科学研究部, 産学官連携研究員 (50418828)
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| Project Period (FY) |
2010 – 2012
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| Keywords | TNNI3K / 心筋トロポニン I / 遺伝子機能 / ELISA / 心筋細胞分化 |
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| Research Abstract |
TNNI3K is a MAP kinase specifically and continuouslyexpressed in cardiac muscle and interacted with cardiac troponin I (TNNI3, or: cTnI). The aim of this study is to investigate role of TNNI3K interacting with cTnI on modification the physiological function of cardiomyocytes, suppressing some pathological injuries and to explain the new molecular mechanism for TNNI3K-mutant in the ischemic coronary heart diseases, and hope to develop some new therapeutic strategies. In the in vitro experiments, 1) Effects of PKC inhibitor (GF109203X) or PKA activator (Br-8-cAMP) on the beating frequency were investigated in P19CL6-derived culture beating cardiomyocytes. Results showed that TNNI3K-overexpression increased the contractility and beating frequency together with restrained phosphorylation of cTnI through activation of the PKA pathway but not by blocking of the PKC pathway. 2) Availability of plasma TNNI3K level was investigated using with anti-TNNI3K poly-antibodies in patients diagnosed as AMI (n=40), chronic heart failure (CHF, n=18) and acute renal failure (ARF, n=6); and in healthy volunteers (n=12). Data shown that circulating TNNI3K levels were significantly higher in AMI (p
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[Journal Article] Lai ZF, Chen YZ, Chen J, Kitamoto Y, Sakaguchi N Kuwahara K, Tsuda H, Sun H, Yang ZQ, Chu JY, Li JM, Zheng JL, and Kim-Mitsuyama S2013
Author(s)
Lai ZF, Chen YZ, Chen J, Kitamoto Y, Sakaguchi N Kuwahara K, Tsuda H, Sun H, Yang ZQ, Chu JY, Li JM, Zheng JL, and Kim-Mitsuyama S
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Journal Title
TNNI3K, as a new diagnosis biomarker for acute myocardial infarction 2013
Peer Reviewed
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[Journal Article] Aliskiren enhances protective effects of valsartan against type 2 diabeti nephropathy in mice2010
Author(s)
Dong YF, Liu L, Lai ZF, Yamamoto E, Kataoka K, Nakamura T, Fukuda M, Tokutomi Y, Nako H, Ogawa H, Kim-Mitsuyama S
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Journal Title
J Hypertens
Volume: 28(7)
Pages: 1554-65
DOI
Peer Reviewed
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