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2012 Fiscal Year Final Research Report

Chemical genomics approach to human embryonic stem cell-self renewal

Research Project

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Project/Area Number 22590269
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General medical chemistry
Research InstitutionKyoto University

Principal Investigator

KAWASE Eihachiro  京都大学, 再生医科学研究所, 講師 (70402790)

Project Period (FY) 2010 – 2012
KeywordsヒトES細胞 / 化合物ライブラリ / 未分化維持 / 分化制御
Research Abstract

Human embryonic stem cells (hESCs) and induced pluripotent cells have the potential to provide an unlimited source of tissues for regenerative medicine. For this purpose, development of defined/xeno-free culture systems under feeder-free conditions is essential for the expansion of hESCs. Most defined/xeno-free media for the culture of hESCs contain basic fibroblast growth factor (bFGF). Therefore, bFGF is thought to have an almost essential role for the expansion of hESCs in an undifferentiated state. Here, we report identification of small molecules, some of which were neurotransmitter antagonists (trimipramine and ethopropazine), which promote long-term hESC self-renewal without bFGF in the medium. The hESCs maintained high expression levels of pluripotency markers, had a normal karyotype after 20 passages, and could differentiate into all three germ layers.

  • Research Products

    (22 results)

All 2013 2012 2011 2010 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (12 results) Book (2 results) Remarks (2 results)

  • [Journal Article] Identification of small molecules that promote human embryonic stem cell self-renewal.2013

    • Author(s)
      Kumagai, H., Suemori, H., Uesugi, M., Nakatsuji, N. and Kawase, E
    • Journal Title

      Biochem. Biophys. Res. Commun

      Volume: 434 Pages: 710-716

    • DOI

      DOI:10.1016/j.bbrc.2013.03.061

    • Peer Reviewed
  • [Journal Article] Recombinant E8 fragments of human laminin isoforms support long-term culture of human pluripotent stem cells under defined and xeno-free culture conditions.2012

    • Author(s)
      Miyazaki, T., Futaki, S., Suemori, H., Taniguchi,Y., Kawasaki, M., Hayashi, M., Kumagai,H., Nakatsuji, N., Sekiguchi, K. and Kawase, E
    • Journal Title

      Nature Communications

      Volume: 13 Pages: 1236

    • DOI

      DOI:10.1038/ncomms2243

    • Peer Reviewed
  • [Journal Article] Efficient and Accurate Homologous Recombination in hESCs and hiPSCs Using Helper-Dependent Adenoviral Vectors2012

    • Author(s)
      Aizawa, E., Hirabayashi,Y.. Iwanaga, Y., Suzuki, K., Sakurai, K. Shimoji, M., Aiba, K., Wada, T., Tooi, N., Kawase, E., Suemori, H., Nakatsuji, N.and Mitani, K
    • Journal Title

      Molecular Therapy

      Volume: 20 (2) Pages: 424-431

    • DOI

      DOI:10.1038/mt.2011.266

    • Peer Reviewed
  • [Journal Article] Cardiomyocytes develop from anterior primitive streak cells induced by β-catenin activation and the blockage of BMP signaling in hESCs.2010

    • Author(s)
      Yamauchi, K., Sumi, T., Minami, I., Otsuji, T.G., Kawase, E., Nakatsuji, N., Suemori, H
    • Journal Title

      Genes to Cells

      Volume: 15 (12) Pages: 1216-1227

    • DOI

      DOI:10.1111/j.1365-2443.2010.01455.x

    • Peer Reviewed
  • [Journal Article] Role of SOX2 in maintaining pluripotency of human embryonic stem cells2010

    • Author(s)
      Adachi, K., Suemori, H., Yasuda, S. -Y., Nakatsuji N. and Kawase, E
    • Journal Title

      Genes to Cells

      Volume: 15 (5) Pages: 455-469

    • Peer Reviewed
  • [Journal Article] Efficient system to integrate functional genes into a defined locus in human embryonic stem cell lines2010

    • Author(s)
      Sakurai, K., Shimoji, M., Tahimic, CGT., Aiba, K., Kawase, E., Hasegawa, K, Amagai, Y., Suemori, H., Nakatsuji, N
    • Journal Title

      Nucleic Acids Res

      Volume: 38 (7) Pages: e76

    • Peer Reviewed
  • [Presentation] ラミニンE8フラグメントを用いたヒトES/iPS細胞の単一分散培養法2013

    • Author(s)
      宮崎隆道、二木杉子、末盛博文、山田雅司、川崎美和、林麻利亜、熊谷英明、中辻憲夫、関口清俊、川瀬栄八郎
    • Organizer
      第12回日本再生医療学会総会
    • Place of Presentation
      横浜
    • Year and Date
      2013-03-23
  • [Presentation] Fluorescent Chemical Probes for Human Stem Cells2013

    • Author(s)
      Nao Hirata, Masato Nakagawa, Yuto Fujibayashi, Kaori Yamauchi, Asako Murata,Itsunari Minami, Maiko Tomioka, Takayuki Kondo, Ting-Fang Kuo, Hiroshi Endo,Haruhisa Inoue, Shin-ichi Sato, Shin Ando,Yoshinori Kawazoe, Kazuhiro Aiba, Koh
    • Organizer
      Cira International Symposium
    • Place of Presentation
      Kyoto
    • Year and Date
      2013-03-11
  • [Presentation] RECOMBINANT HUMAN LAMININ E8 FRAGMENTS (LM-E8s) SUPPORT THE EFFICIENT ADHESION AND EXPANSION OF DISSOCIATED HUMAN PLURIPOTENT STEM CELLS UNDER DEFINED AND XENO-FREE CONDITION2012

    • Author(s)
      Takamichi Miyazaki, Sugiko Futaki, Hirofumi Suemori, Yukimasa Taniguchi, Masashi Yamada, Miwa Kawasaki, Maria Hayashi, Hideaki Kumagai, Norio Nakatsuji, Kiyotoshi Sekiguchi, Eihachiro Kawase
    • Organizer
      2012 World Stem Cell Summit
    • Place of Presentation
      West Palm Beach, USA
    • Year and Date
      2012-12-03
  • [Presentation] Recombinant E8 fragments of human laminin isoforms support the efficient adhesion and expansion of dissociated human pluripotent stem cells under defined and xeno-free condition.2012

    • Author(s)
      Takamichi Miyazaki, Sugiko Futaki, Hirofumi Suemori, Yukimasa Taniguchi, Masashi Yamada, Miwa Kawasaki, Maria Hayashi, Hideaki Kumagai, Norio Nakatsuji, Kiyotoshi Sekiguchi, Eihachiro Kawase
    • Organizer
      10thInternational Society for Stem Cell Research
    • Place of Presentation
      Yokohama
    • Year and Date
      2012-06-15
  • [Presentation] Manufacturing and banking of clinical-garade human embryonic stem cell lines in a GMP facility2012

    • Author(s)
      Takada, Kei, Hirai, Masako, Kawase, Eihachiro, Hamao, Mari, Kashigi, Fumi, Suemori, Hirofumi, Nakatsuji, Norio, Takahashi, Tsuneo A
    • Organizer
      10thInternational Society for Stem Cell Research
    • Place of Presentation
      Yokohama
    • Year and Date
      2012-06-15
  • [Presentation] Applications of scalable culture system for clinical-grade undifferentiated human embryonic stem cells.2012

    • Author(s)
      Kawase E
    • Organizer
      Wednesday Industry Symposia, 10thInternational Society for Stem Cell Research
    • Place of Presentation
      Yokohama
    • Year and Date
      2012-06-13
  • [Presentation] Fluorescent chemical probes for human stem cells2011

    • Author(s)
      Nao Hirata,Masato Nakagawa,Yuto Fujibayashi, Kaori Yamauchi, Asako Murata, Eihachiro Kawase, Shin-ichi Sato, Shin Ando,Young-Tae Chang, Hirofumi Suemori,Norio Nakatsuji,Kazumitsu Ueda, Shinya Yamanaka, Motonari Uesugi.
    • Organizer
      AIMECS11 8thAFMC International Medical Chemistry Symposium
    • Place of Presentation
      Tokyo
    • Year and Date
      2011-11-29
  • [Presentation] Homologous Recombination in human pluripotent stem cells using helper-dependent adenovirua vectors2011

    • Author(s)
      Suzuki, K., Hirabayashi, Y., Aizawa, E., Iwanaga, Y., Tokumitsu, A., Sakurai, K., Shimoji, M., Aiba, K., Wada, T., Tooi, N., Kawase, E. Suemori, H., Nakatsuji, N. and Mitani, K
    • Organizer
      9thInternational Society for Stem Cell Research
    • Place of Presentation
      Toronto, Canada
    • Year and Date
      2011-06-17
  • [Presentation] ヒトES細胞の未分化維持因子の探索を2011

    • Author(s)
      熊谷英明、末盛博文、上杉志成、中辻憲夫、川瀬栄八郎
    • Organizer
      第10回日本再生医療学会総会
    • Place of Presentation
      東京
    • Year and Date
      2011-03-01
  • [Presentation] β-cateninの活性化およびBMPシグナルの抑制による原条前側領域の形成を経由したヒトES細胞から心筋細胞への分化2010

    • Author(s)
      山内香織、角智行、南一成、尾辻智美、川瀬栄八郎、中辻憲夫、末盛博文
    • Organizer
      第33回日本分子生物学会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-10
  • [Presentation] High Content Analysis (HCA)によるヒトES細胞の未分化維持因子の探索2010

    • Author(s)
      熊谷英明、末盛博文、上杉志成、中辻憲夫、川瀬栄八郎
    • Organizer
      High Content Analysis (HCA)によるヒトES細胞の未分化維持因子の探索
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-10
  • [Presentation] Gene targeting in human pluripotent stem cells with adeno-associated virus vectors2010

    • Author(s)
      Ko Mitani, Kaoru Mitsui, Keiichiro Suzuki, Emi Aizawa, Haruka Shiiba, Eihachiro Kawase, Hirofumi Suemori, Norio Nakatsuji
    • Organizer
      第16回日本遺伝子治療学会
    • Place of Presentation
      栃木
    • Year and Date
      2010-07-01
  • [Book] 実験医学4月号2013

    • Author(s)
      川瀬栄八郎、宮崎隆道、関口清俊
    • Total Pages
      31
  • [Book] Stem Cells in Clinic and Research2011

    • Author(s)
      Ali Gholamrezanezhad
    • Publisher
      InTech
  • [Remarks] 細胞接着タンパクを用い安全・高効率なヒトES/iPS細胞の培養法を開発

    • URL

      http://www.icems.kyoto-u.ac.jp/j/pr/2012/12/05-nr.html

  • [Remarks] 細胞接着タンパクを用い、 安全・高効率なヒトES/iPS細胞の培養法を開発

    • URL

      http://www.kyoto-u.ac.jp/ja/news_data/h/h1/news6/2012/121205_1.htm

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Published: 2014-08-29   Modified: 2015-10-07  

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