Advanced investigation of molecular regulation of lipid metabolism by the transcription factor Nrf1

2011 Fiscal Year Final Research Report

• Project/Area Number: 22590277
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General medical chemistry
• Research Institution: Doshisha University
• Principal Investigator: KOBAYASHI Akira 同志社大学, 生命医科学部, 教授 (50292214)
• Co-Investigator(Kenkyū-buntansha): TSUCHIYA Yoshiki 同志社大学, 生命医科学部, 助教 (30456777)
• Project Period (FY): 2010 – 2011
• Keywords: 脂質代謝異常 / 遺伝子発現制御 / タンパク質分解機構 / プロテアソーム

Research Abstract

The experimental purpose of this project is to investigate regulatory mechanisms of the transcription factor Nrf1(NF-E2-related factor 1) for lipid metabolism. First, we found that the turnover of Nrf1 is regulated by the ubiquitin ligase Hrd1 and β-TrCP in the cytoplasm and nucleus, respectively. Second, we identified that proteasome subunit genes psmc4 and psmb6 were direct Nrf1 target genes.

Research Products

• [Journal Article] Dual regulation of the transcriptional activity of Nrf1 by β-TrCP-and Hrd1-dependent degradation mechanisms (2011)
  • Author(s): Tsuchiya, Y., Morita, T., Kim, M., Iemura, S., Natsume, T., Yamamoto, M. and Kobayashi, A.
  • Journal Title: Mol. Cell. Biol. Volume: 31 Pages: 4500-4512
  • Peer Reviewed
• [Journal Article] Central nervous system-specific deletion of transcription factor Nrf1 causes progressive motor neuronal dysfunction (2011)
  • Author(s): Kobayashi, A., Tsukide, T., Miyasaka, T., Morita, T., Mizoroki, T., Saito, Y., Ihara, Y., Takashima, A., Noguchi, N., Fukamizu, A., Hirotsu, Y., Ohtsuji, M., Katsuoka, F. and Yamamoto, M.
  • Journal Title: Genes Cells Volume: 16 Pages: 692-703
  • Peer Reviewed
• [Presentation] Dual regulation of the transcriptional activity of Nrf1 by β-TrCP-and Hrd1-dependent degradation mechanisms (2011)
  • Author(s): 土谷佳樹, 森田智子, 金美姫, 家村俊一郎, 夏目徹, 山本雅之, 小林聡
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20111213-16
• [Presentation] CK2 regulates the transcriptional activity of Nrf1 to maintain cellular homeostasis (2011)
  • Author(s): 伊藤義起, 土谷佳樹, 家村俊一郎, 夏目徹, 小林聡
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20111213-16
• [Presentation] Dual regulation of the transcription factor Nrf1(Nfe2l1) by β-TrCP-and Hrd1-dependent degradation mechanisms (2011)
  • Author(s): Tsuchiya, Y., Morita, T., Kim, M., Iemura, S., Natsume, T., Yamamoto, M., and Kobayashi, A.
  • Organizer: EMBO Workshop on Intracellular Proteolysis and Cancer
  • Place of Presentation: Valencia, Spain
  • Year and Date: 20111026-28
• [Presentation] Cytoplasmic and nuclear degradation mechanisms regulate biological function of the transcription factor Nrf1 (2010)
  • Author(s): Morita, T., Tsuchiya, Y., Kim, M., Iemura, S., Tsukide, T., Natsume, T., Yamamoto, M., and Kobayashi, A.
  • Organizer: 第33回日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 20101207-10
• [Presentation] Central nervous system-specific deletion of transcription factor Nrf1causes progressive motor neuronal dysfunction (2010)
  • Author(s): Tsukide, T., Kobayashi, A., Miyasaka, T. Morita, T., Mizoroki, T., Saito, Y., Ihara, Y. Takashima, A., Noguchi, N. Fukamizu, A., Hirotsu, Y., Ohtsuji, M., Katsuoka, F. and Yamamoto M
  • Organizer: 5th International Symposium on GATA transcription factor
  • Place of Presentation: Sendai, Japan
  • Year and Date: 20101117-19
• [Presentation] CK2 regulates the transcription factor Nrf1 to maintain cellular homeostasis (2010)
  • Author(s): Ito, Y., Tsuchiya, Y., Iemura, S., Natsume, T., and Kobayashi, A.
  • Organizer: 6th International Conference on Protein Kinase CK2
  • Place of Presentation: Cologne, Germany
  • Year and Date: 20100907-10
• [Presentation] リン酸化による転写因子Nrf1の機能制御機構解析 (2010)
  • Author(s): 伊藤義起, 土谷佳樹, 家村俊一郎, 夏目徹, 小林聡
  • Organizer: 分子生物学会第10回春季シンポジウム
  • Place of Presentation: 松島,宮城
  • Year and Date: 20100607-08
• [Remarks]
  • URL: http://biomedical.doshisha.ac.jp/staff/index.html#gaku_ise