2012 Fiscal Year Final Research Report
Recruitment of Polycomb-group complex for stable repressions
| Project/Area Number |
22590278
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
General medical chemistry
|
|---|
| Research Institution | The Institute of Physical and Chemical Research |
|---|
Principal Investigator |
ISONO Kyoichi 独立行政法人理化学研究所, 免疫器官形成研究グループ, 上級研究員 (90323435)
|
|---|
| Project Period (FY) |
2010 – 2012
|
| Keywords | ポリコーム / 発現制御 |
|---|
| Research Abstract |
To better understand recruitment mechanisms of PRC1, we focused on subnuclear domains consisting of PRC1. To data H3K27me3 is known to be one of factors for PRC1 recruitment. Conditional deletion experiments of Ezh2 however did not support that H3K27me3 was a critical recruiter of PRC1. On the other hand, FRAP analysis showed that about 20% of PRC1 component was immobile on chromatin, implying that such an immobile fraction becomes a memory tag of PRC1 beyond cell division. We thus have been addressing this hypothesis
|
