Neural differentiation dysfunction in cells with autosomal imbalance

2012 Fiscal Year Final Research Report

• Project/Area Number: 22590301
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Human genetics
• Research Institution: Tottori University
• Principal Investigator: HIRATSUKA Masaharu 鳥取大学, 医学部, 助教 (00362872)
• Project Period (FY): 2010 – 2012
• Keywords: ゲノム医科学

Research Abstract

Although particular chromosomal syndromes are phenotypically and clinically distinct, the majority of individuals with autosomal imbalance, such as Down syndrome, manifest mental retardation. To study the developmental stages of complex neuronal diseases, several independent disomic and trisomic human ES (T21-hES) and iPS (DS-iPS) cell lines were generated and subsequently differentiated into neuronal progenitor cells. Whereas T21-ES and DS-iPS cells generated neurons similarly to disomic control cells, expression pattern of neural differentiation markers in these cells was different from that of controls.

Research Products

• [Journal Article] Prognostic significance of sirtuin 2 protein nuclear localization in glioma: an immunohistochemical study (2012)
  • Author(s): Imaoka N, Hiratsuka M, Osaki M, Kamitani H, Kambe A, Fukuoka J, Kurimoto M, Nagai S, Okada F, Watanabe T, Ohama E, Kato S, Oshimura M
  • Journal Title: Oncol. Rep Volume: 28 Pages: 923-930
  • DOI: DOI:10.3892/or.2012.1872
  • Peer Reviewed
• [Journal Article] Integration-free iPS cells engineered using human artificial chromosome vectors (2011)
  • Author(s): Hiratsuka MD S-i PS 6 -1 2ectoderm mesoderm endodermD S-i PS 8 -3図3 ダウン iPS 細胞の in vivo 3 胚葉分化, Uno N, Ueda K, Kurosaki H, Imaoka N, Kazuki K, Ueno E, Akakura Y, Katoh M, Osaki M, Kazuki Y, Nakagawa M, Yamanaka S, Oshimura M.
  • Journal Title: PLoS One Volume: 6 Pages: e25961
  • DOI: DOI:10.1371/journal.pone.0025961
  • Peer Reviewed
• [Journal Article] Refined human artificial chromosome vectors for gene therapy and animal transgenesis (2011)
  • Author(s): Kazuki K, Hoshiya H, Takiguchi M, Abe S, Iida Y, Osaki M, Katoh M, HiratsukaM, Shirayoshi Y, Hiramatsu K, Ueno E, Kajitani N, Yoshino T, Kazuki K, Ishihara C, Takehara S, Tsuji S, Ejima F, Toyoda A, Sakaki Y, Larionov V, Kouprina N, Oshimura M
  • Journal Title: Gene The Volume: 18 Pages: 384-93
  • DOI: DOI:10.1038/gt.2010.147
  • Peer Reviewed
• [Presentation] 染色体導入技術を用いた同一遺伝的背景を持つトリソミーヒト ES 細胞の作製 (2012)
  • Author(s): 香月康宏
  • Organizer: 日本人類遺伝学会第57回大会
  • Place of Presentation: 東京
  • Year and Date: 2012-10-27
• [Presentation] ヒト人工染色体ベクターを用いたiPS細胞の作製 (2011)
  • Author(s): 平塚正治
  • Organizer: 第10回日本再生医療学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-03-02