The analyses of tight junction formation via disulfide bond

2012 Fiscal Year Final Research Report

• Project/Area Number: 22590336
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Human pathology
• Research Institution: Sapporo Medical University
• Principal Investigator: TANAKA Satoshi 札幌医科大学, 医学部, 講師 (30374250)
• Project Period (FY): 2010 – 2012
• Keywords: 分子病理 / タイト結合 / ジスルフィド結合

Research Abstract

We found that occludin, a transmembrane protein found in tightjunctions, had an intra-molecular disulfide bond in its cytoplasmic tail of the c-terminus and that the ratio of disulfide bond formation depended on its sub-cellular location. In addition, we discovered that a disulfide bond in the extracellular loop of occludin might affect its stability. Co-immunoprecipitation with occludin containing a cysteine mutation revealed an associated with some kinds of protein thatmight regulate disulfide bond formation. We also found that zo-1, another tight junctionprotein, might have dimerized via a disulfide bond.

Research Products

• [Journal Article] Behavior of tricellulin during destruction and formation of tight junctions under various extracellular calcium conditions (2013)
  • Author(s): Takasawa A, Kojima T, Ninomiya T, Tsujiwaki M, Murata M, Tanaka S, Sawada N
  • Journal Title: Cell Tissue Res Volume: 351(1) Pages: 73-84
  • DOI: DOI:10.1007/s00441-012-1512-7
  • Peer Reviewed
• [Journal Article] An undifferentiated embryonal sarcoma of the liver containing adipophilin-positive vesicles in an adult with massive sinusoidal invasion (2012)
  • Author(s): Tanaka S*, Takasawa A, Fukasawa Y, Hasegawa T, Sawada N
  • Journal Title: Int J Clin Exp Pathol Volume: 5(8) Pages: 824-829
  • URL: http://www.ijcep.com/files/ijcep1207003.pdf
  • Peer Reviewed
• [Journal Article] Epidermal growth factor modulates claudins and tight junctional functions in ovarian cancer cell lines (2012)
  • Author(s): Ogawa M, Kojima T, Someya M, Nomura K, Takasawa A, Murata M, Tanaka S, Saito T, Sawada N
  • Journal Title: Histochem Cell Biol Volume: 138(2) Pages: 323-338
  • DOI: DOI:10.1007/s00418-012-0956-x
  • Peer Reviewed
• [Journal Article] Expression of claudins 7 and 18 in pancreatic ductal adenocarcinoma: association with features of differentiation (2012)
  • Author(s): Soini Y, Takasawa A, Eskelinen M, Juvonen P, Karja V, Hasegawa T, Murata M, Tanaka S, Kojima T, Sawada N
  • Journal Title: J Clin Pathol Volume: 65(5) Pages: 431-436
  • DOI: DOI:10.1136/jclinpath-2011-200400
  • Peer Reviewed
• [Journal Article] Claudin-4-targeted therapy using Clostridium perfringens enterotoxin for prostate cancer (2012)
  • Author(s): Maeda T, Murata M, Chiba H, Takasawa A, Tanaka S, Kojima T, Masumori N, Tsukamoto T, Sawada N
  • Journal Title: Prostate Volume: 72(4) Pages: 351-360
  • DOI: DOI:10.1002/pros.21436
  • Peer Reviewed
• [Journal Article] Protein kinase Calpha inhibitor enhances the sensitivity of human pancreatic cancer HPAC cells to Clostridium perfringens enterotoxin via claudin-4 (2011)
  • Author(s): Kyuno D, Kojima T, Ito T, Yamaguchi H, Tsujiwaki M, Takasawa A, Murata M, Tanaka S, Hirata K, Sawada N
  • Journal Title: Cell Tiisue Res Volume: 346(3) Pages: 369-81
  • DOI: DOI:10.1007/s00441-011-1287-2
  • Peer Reviewed
• [Journal Article] Downregulation of tight junction-associated MARVEL protein marvelD3 during epithelial-mesenchymal transition in human pancreatic cancer cells (2011)
  • Author(s): Kojima T, Takasawa A, Kyuno D, Ito T, Yamaguchi H, Hirata K, Tsujiwaki M, Murata M, Tanaka S, Sawada N
  • Journal Title: Exp Cell Res Volume: 317(16) Pages: 2288-2298
  • DOI: DOI:10.1016/j.yexcr.2011.06.020
  • Peer Reviewed
• [Journal Article] An NF-・B signaling pathway via PKC・regulates replication of respiratory syncytial virus in polarized normal human nasal epithelial cells (2011)
  • Author(s): Masaki T, Kojima T, Okabayashi T, Ogasawara N, Ohkuni T, Obata K, Takasawa A, Murata M, Tanaka S, Hirakawa S, Fuchimoto J, Ninomiya T, Fujii N, Tsutsumi H, Himi T, Sawada N
  • Journal Title: Mol Biol Cell Volume: 22(13) Pages: 2144-2156
  • DOI: DOI:10.1091/mbc.E10-11-0875
  • Peer Reviewed
• [Journal Article] Transcriptional regulation of claudin-18 via specific protein kinase C signaling pathways and modification of DNA methylation in human pancreatic cancaer cells (2011)
  • Author(s): Ito T, Kojima T, Yamaguchi H, Kyuno D, Kimura Y, Imamura M, Takasawa A, Murata M, Tanaka S, Hirata K, Sawada N
  • Journal Title: J Cell Biochem Volume: 112(7) Pages: 1761-72
  • DOI: DOI:10.1002/jcb.23095
  • Peer Reviewed
• [Journal Article] Poly(I:C) reduces expression of JAM-A and induces secretion of IL-8 and TNF-・via distinct NF-・B pathways in human nasal epithelial cells (2011)
  • Author(s): Ohkuni T, Kojima T , Ogasawara N, Masaki T, Fuchimoto J, Kamekura R, Koizumi J, Ichimiya S, Murata M, Tanaka S, Himi T, Sawada N
  • Journal Title: Toxicology and Applied Pharmacology Volume: 250 Pages: 29-38
  • DOI: DOI:10.1016/j.taap.2010.09.023
  • Peer Reviewed
• [Journal Article] Transcriptional control of tight junction proteins via a protein kinase C signal pathway in human telomerase reverse transcriptase-transfected human pancreatic duct epithelial cells (2010)
  • Author(s): Yamaguchi H, Kojima T, Ito T, Kimura Y, Imamura M, Son S, Koizumi J, Murata M, Nagayama M, Nobuoka T,Tanaka S,Hirata K, Sawada N
  • Journal Title: Am J Pathol Volume: 177 Pages: 698-712
  • DOI: DOI:10.2353/ajpath.2010.091226
  • Peer Reviewed
• [Journal Article] PPARγ agonists upregulate the barrier function of tight junctions via a PKC pathway in human nasal epithelial cells (2010)
  • Author(s): Ogasawara N, Kojima T, Go M, Ohkuni T, Koizumi J, Kamekura R, Masaki T, Murata M, Tanaka S,Fuchimoto J, Himi T, Sawada N
  • Journal Title: Pharmacol Res Volume: 61 Pages: 489-498
  • DOI: DOI:10.1016/j.phrs.2010.03.002
  • Peer Reviewed
• [Journal Article] Protein kinase Calpha inhibitor protects against downregulation of claudin-1 during epithelial-mesenchymal transition of pancreatic cancer
  • Author(s): Kyuno D, Kojima T, Yamaguchi H, Ito T, Kimura Y, Imamura M, Takasawa A, Murata M, Tanaka S, Hirata K, Sawada N
  • Journal Title: Carcinogenesis
  • Peer Reviewed
• [Presentation] 胆道癌における claudin-18、maspin の免疫組織化学的検討 (2012)
  • Author(s): 高澤啓、計良淑子、村田雅樹、荻野次郎、及能大輔、田中敏、小島隆、長谷川匡、澤田典均
  • Organizer: 第101回日本病理学会総会
  • Place of Presentation: 東京
  • Year and Date: 2012-04-28
• [Presentation] クローディン-6による細胞間接着は幹細胞の上皮分化を誘導する (2012)
  • Author(s): 杉本幸太郎、冨川直樹、金居李紗、田中敏、杉野隆、澤田典均、千葉英樹
  • Organizer: 第101回日本病理学会総会
  • Place of Presentation: 東京
  • Year and Date: 2012-04-28
• [Presentation] 膵癌組織および膵癌細胞株におけるタイト結合分子tricellulin の核への局在 (2012)
  • Author(s): 村田雅樹、小野佑輔、高澤啓、田中敏、小島隆、澤田典均
  • Organizer: 第101回日本病理学会総会
  • Place of Presentation: 東京
  • Year and Date: 2012-04-27
• [Presentation] 肝炎および肝癌におけるタイト結合分子の発現変化 (2012)
  • Author(s): 辻脇光洋、村田雅樹、高澤啓、平塚佑太郎、福田利英子、田中敏、小島隆、澤田典均
  • Organizer: 第101回日本病理学会総会
  • Place of Presentation: 東京
  • Year and Date: 2012-04-27
• [Presentation] 卵巣癌細胞株におけるタイト結合分子claudin-4 に対する CPE 作用条件についての検討 (2011)
  • Author(s): 井上万梨絵、田中敏、染谷真行、高澤啓、村田雅樹、小島隆、斎藤豪、澤田典均
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2011-04-30
• [Presentation] タイト結合分子クローディンによる幹細胞の上皮分化誘導機構 (2011)
  • Author(s): 杉本幸太郎、冨川直樹、田中敏、杉野隆、澤田典均、千葉英樹
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2011-04-28
• [Presentation] 培養ヒト膵管上皮細胞における tricellulin 発現調節機構の解明 -Ca スイッチによる発現・局在変化を中心に (2011)
  • Author(s): 高澤啓、小島隆、及能大輔、辻脇光洋、村田雅樹、田中敏、澤田典均
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2011-04-28
• [Presentation] 膵癌細胞株及び正常膵管上皮細胞におけるタイト結合と PKCa との関係 (2011)
  • Author(s): 及能大輔、小島隆、伊東竜哉、山口洋志、辻脇光洋、高澤啓、村田雅樹、田中敏、澤田典均
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2011-04-28
• [Book] 細胞間結合装置、日本臨床分子形態学会編、病気の分子形態学、学際企画 (2011)
  • Author(s): 小島隆、村田雅樹、高澤啓、田中敏、澤田典均
  • Total Pages: 54-57