Biological implication of microRNAs in tumorigenesis, tumor development, and progression ? analysis with in situ hybridization using histopathological sections

2012 Fiscal Year Final Research Report

• Project/Area Number: 22590339
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Human pathology
• Research Institution: Fujita Health University
• Principal Investigator: INADA Kenichi 藤田保健衛生大学, 医学部, 准教授 (70246081)
• Co-Investigator(Kenkyū-buntansha): 水谷 泰嘉 藤田保健衛生大学, 医学部, 助教 (10546229)
• Project Period (FY): 2010 – 2012
• Keywords: microRNA / in situ hybridization / LNA/DNA オリゴプローブ / thyramide / 病理組織切片 / 食道扁平上皮癌 / Brm / Egr1

Research Abstract

Although we examined various tumors including esophageal cancer (squamous cell carcinoma), gastric cancer (adenocarcinoma), ovarian cancer (adenocarcinoma), and hepatocellular carcinoma in this study, no apparent biological interrelationship between microRNAs and their targeted specific proteins was revealed except esophageal cancer. We supposed that the central reason for the failure to detect the interrelationship was deterioration of ISH probes and unexpected change of company that supplied them. On the contrary, we found that microRNA 199a, Brm, and Egr1 constructed double-negative feed in esophageal cancer, suggesting that they were mutually related in the process of esophageal carcinogenesis, cancer development, and its progression. But no special relationship was found between them and tumor stage. We are continuously studying about other tumors both epithelial and nonepithelial and various precancerous lesions because this research method is very unique.

Research Products

• [Journal Article] Novel approach to identifying autoantibodies in rheumatoid synovitis with a biotinylated human autoantigen library and the enzyme-labeled method. (2012)
  • Author(s): Mizutani Y., Matsuoka K., Takeda H.,Shiogama K., Inada K., Hayakawa K.,Yamada H., Miyazaki T., Sawasaki T.,Endo Y., Tsutsumi Y.
  • Journal Title: J. Immunol. Method. Volume: 387 Pages: 57-70
  • Peer Reviewed
• [Journal Article] Multiple microRNAs induced by Cdx1 suppress Cdx2 in human colorectal tumour cells. (2012)
  • Author(s): Tagawa T., Haraguchi T., Hiramatsu H., Kobayashi K., Sakurai K., Inada K., Iba H.
  • Journal Title: Biochem.J Volume: 447 Pages: 449-455
  • Peer Reviewed
• [Journal Article] Rebamipide induces dendritic cell recruitment to N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-exposed rat gastric mucosa based on IL-1β upregulation. (2012)
  • Author(s): Yamamichi N., Oka M., Inada K., Konno-Shimizu M., Kageyama-Yahara N., Tamai H., Kato J., Fujishiro M., Kodashima S., Niimi K., Ono S.,Tsutsumi Y., Ichinose M., Koike K.
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 424 Pages: 124-129
  • Peer Reviewed
• [Journal Article] Specific in situ visualization of plasma cells producing antibodies against porphyromonasgingivalis in gingival radicular cyst.Application of the enzyme-labeled antigen method. (2011)
  • Author(s): Tsuge S., Mizutani Y., Matsuoka K., Sawasaki T., Endo Y., Naruishi K., Maeda H., Takashiba S., Shiogama K., Inada K., Tsutsumi Y.
  • Journal Title: J. Histochem. Cytochem Volume: 59 Pages: 673-689
  • Peer Reviewed
• [Journal Article] MicroRNAs miR-199a-5p and -3p target the Brm subunit of SWI/SNF to generate a double-negative feedback loop in a variety of human cancers. (2011)
  • Author(s): Sakurai K., Furukawa C., Haraguchi T., Inada K., Shiogama K., Tagawa T., Fujita S., Ueno Y., Ogata A., Ito M., Tsutsumi Y., Iba H.
  • Journal Title: Cancer Res Volume: 71 Pages: 1680-1689
  • Peer Reviewed
• [Presentation] 他miR-199a-5p と-3p は,エピジェネティックスに重要な因子である Brm を標的とする (2010)
  • Author(s): 櫻井浩平,稲田健一,伊庭英夫
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪
  • Year and Date: 2010-09-24