MG23-mediatedcrosstalk between genetic system and immune system.

2012 Fiscal Year Final Research Report

• Project/Area Number: 22590359
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: The University of Tokushima
• Principal Investigator: YAMAZAKI Tetsuo 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授 (90330208)
• Project Period (FY): 2010 – 2012
• Keywords: 小胞体 / 細胞死 / シグナル伝達

Research Abstract

The endoplasmic reticulum (ER) operates in adaptive responses to various stresses, dictating cell fate. Here we show thatknockdown of the ER protein mitsugumin23 (MG23) enhances cell death induced by ultraviolet C (UVC), which causes DNA damage. The small heat shock protein αB-crystallin (αBC) is identified as a MG23 binding molecule and its knockdown facilitates death of UVC-exposed cells. Conversely, αBC lowered UVC sensitivity when expressed as an ER-anchored form. Taken together, the results suggest that MG23plays a protective role against UVC by accumulating αBC in the close vicinity of the ER.

Research Products

• [Journal Article] Protective role of the endoplasmic reticulum protein mitsugumin23 against ultraviolet C-induced cell death. (2013)
  • Author(s): Yamashita Arisa, Taniwaki Tatsuya, Kaikoi Yuka, Yamazaki Tetsuo
  • Journal Title: FEBS Lett Volume: Vol.587 Pages: 1299-1303
  • DOI: doi:10.1016/j.febslet.2013.03.024
  • Peer Reviewed
• [Journal Article] Pasare Chandrashekhar, Role for B-cell adapter for PI3K (BCAP) as a signaling adapter linking Toll-like receptors (TLRs) to serine/threonine kinases PI3K/Akt. (2012)
  • Author(s): Troutman Ty Dale, Hu Wei, Fulenchek Stephanie, Yamazaki Tetsuo, Kurosaki Tomohiro, Bazan J Fernando
  • Journal Title: Proc Natl Acad Sci USA Volume: Vol.109 Pages: 273-278
  • DOI: doi:10.1073/pnas.1118579109
  • Peer Reviewed
• [Journal Article] Kurosaki Tomohiro, Greenberg Steven, A Requirement for the p85 PI3K Adapter Protein BCAP in the Protection of Macrophages from Apoptosis Induced by Endoplasmic Reticulum Stress (2011)
  • Author(s): Song Sungwon, Chew Claude, Dale M. Benjamin, Traum Daniel, Peacock James, Yamazaki Tetsuo, Clynes Raphael, Kurosaki Tomohiro, Greenberg Steven
  • Journal Title: J Immunol Volume: Vol.187 Pages: 619-625
  • DOI: doi:10.4049/jimmunol.0903425
  • Peer Reviewed
• [Presentation] ケミカルツールを用いたsiRNA-タンパク質間の相互作用様式解明 (2013)
  • Author(s): 南川典昭
  • Organizer: 第133年会日本薬学会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 20130328-30
• [Presentation] ミトコンドリア由来のROSシグナルは骨芽細胞の分化を誘導する (2012)
  • Author(s): 山崎哲男
  • Organizer: 第51回日本薬学会・日本薬剤師会・日本病院薬剤師会・中国四国支部学術大会
  • Place of Presentation: 島根県民会館・サンラポーむらくも(松江市)
  • Year and Date: 20121110-11
• [Presentation] 小胞体タンパク質EPIGは紫外線照射による細胞死を抑制する (2012)
  • Author(s): 山崎哲男
  • Organizer: 第51回日本薬学会・日本薬剤師会・日本病院薬剤師会・中国四国支部学術大会
  • Place of Presentation: 島根県民会館・サンラポーむらくも(松江市)
  • Year and Date: 20121110-11
• [Presentation] 小胞体タンパク質EPIGの紫外線応答における役割 (2011)
  • Author(s): 山崎哲男
  • Organizer: 第50回日本薬学会・日本薬剤師会・日本病院薬剤師会・中国四国支部学術大会
  • Place of Presentation: サンポートホール高松・かがわ国際会議場(高松市)
  • Year and Date: 20111112-13
• [Presentation] 小胞体タンパク質mitsugumin23によるDNA損傷性細胞死の促進 (2010)
  • Author(s): 山崎哲男
  • Organizer: 第117回日本薬理学会近畿部会
  • Place of Presentation: 徳島県郷土文化会館(徳島市)
  • Year and Date: 2010-07-08