Functional analysis of motility-related genes of Trypanosoma brucei: relationship with cellular morphology and apoptosis

2012 Fiscal Year Final Research Report

• Project/Area Number: 22590380
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Parasitology (including Sanitary zoology)
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: TAKASHI Suzuki 東京医科歯科大学, 大学院・医歯学総合研究科, 特任教授 (70305530)
• Project Period (FY): 2010 – 2012
• Keywords: アポトシス / 鞭毛 / アフリカトリパノソーマ原虫

Research Abstract

Double knock-down analysis of TbUNC119 and its binding molecule showed cellular apoptosis, morphological change (extended cell shape), and reduced motility in insect-form trypanosome cells. Among those phenotypes, extended cell shape was firstly observed. This was revealed to be at least in part by up-regulated endocytosis. In bloodstream-form trypanosome cells, TbUNC119 was expressed in flagellum and an area around flagella pocket, only in which endocytosis occurred. Functions of TbUNC119 and its binding protein were thus considered to include regulation of cellular endocytosis.

Research Products

• [Journal Article] Differential KineticActivities of Glycerol Kinase among African Trypanosome Species: Phylogenetc and Therapeutic Implications (2011)
  • Author(s): Ohashi-Suzuki M, Yabu Y, Ohshima S, Nakamura K, Kido Y, Sakamoto K, Kita K, Ohta N, Suzuki T
  • Journal Title: J Vet Med Sci Volume: 73(5) Pages: 615-21
  • Peer Reviewed
• [Journal Article] Administrative practices of health professionals and use of artesunate-amodiaquine by community members for treating uncomplicated malaria in southern Ghana: implications for artemisinin-based combination therapy deployment (2011)
  • Author(s): Kwansa-Bentum B, Ayi I, Suzuki T,Otchere J, Kumagai T, Anyan WK, Asahi H, Akao N, Wilson MD, Boakye DA, Ohta N
  • Journal Title: Trop Med Int Health Volume: 10. doi:10.1111n
  • Peer Reviewed
• [Journal Article] Plasmodium falciparum isolates from southern Ghana exhibit polymorphisms in the SERCA-type PfATPase6 though sensitive to artesunate in vitro (2011)
  • Author(s): Kwansa-Bentum B, Ayi I, Suzuki T, Otchere J, Kumagai T, Anyan WK, Osei JH, Asahi H, Ofori MF, Akao N, Wilson MD,Boakye DA, Ohta N
  • Journal Title: Malar J Volume: 10:187
  • Peer Reviewed
• [Journal Article] The biological investigation of prostacyclin in preeclamptic women seen reduced endothelial function (2010)
  • Author(s): Yamamoto T, Suzuki Y, Kojima K, Suzumori N, Suzuki T
  • Journal Title: Hypertens Pregnancy Volume: 29(4) Pages: 484-91
  • Peer Reviewed
• [Presentation] Finding a Novel Drug Target for African Trypanosomiasis (2013)
  • Author(s): Suzuki T
  • Organizer: Asian-AfricanResearch Forum on Emerging andReemerging Infections
  • Place of Presentation: Tokyo (Japan)
  • Year and Date: 2013-01-23
• [Presentation] Molecular cloning and functional analysis of Trypanosoma brucei motility-related genes (2013)
  • Author(s): Suzuki T, Ohshima S, Ohashi-Suzuki M, Dadzie S, Boakye D, Ohta N
  • Organizer: Asian African Research Forum for on Emerging and Reemerging Infections
  • Place of Presentation: Kobe (Japan)
  • Year and Date: 2013-01-11
• [Presentation] Plasmodium falciparum isolates from southern Ghana exhibit polymorphisms in the SERCA-type PfATPase6 though sensitive to artesunate in vitro (2013)
  • Author(s): Kwansa-Bentum B, Ayi I, Suzuki T, Otchere J, Kumagai T, Anyan WK, Osei JH, Asahi H, Ofori MF, Akao N, Wilson MD, Boakye DA, Ohta N
  • Organizer: Asian African Research Forum for on Emerging and Reemerging Infections
  • Place of Presentation: Kobe (Japan)
  • Year and Date: 2013-01-11
• [Presentation] Bio-efficacy, user perception and acceptability of some selected pyrethoid-based mosquito coils in controlling An. gambiae s.l., a malaria vector in some parts of the Greater Accra region of Ghana (2012)
  • Author(s): Dadzie S, Adu-Acheampong S, Kyerematen R, Williams J, Suzuki T, Ohta N, Appawu M, Boakye DA
  • Organizer: Asian African Research Forum for on Emerging and Reemerging Infections
  • Place of Presentation: Kobe (Japan)
  • Year and Date: 2012-01-11
• [Presentation] Detection of Trypanosoma species from Tsetse flies anddomestic animals in the Central and Volta regions of Ghana (2011)
  • Author(s): Alhassan A, Nakayima J, Nakao R, Suzuki T, Sugimoto C
  • Organizer: SATREPS Joint Workshop
  • Place of Presentation: Accra (Ghana)
  • Year and Date: 2011-12-04
• [Presentation] Plasmodium falciparum isolates from southern Ghana exhibit polymorphisms in the SERCA-type PfATPase6 though sensitive to artesunate in vitro (2011)
  • Author(s): Kwansa-Bentum B, Ayi I, Suzuki T, Otchere J, Kumagai T, Anyan WK, Osei JH, Asahi H, Ofori MF, Akao N, Wilson MD, Boakye DA, Ohta N
  • Organizer: SATREPS Joint Workshop
  • Place of Presentation: Accra (Ghana)
  • Year and Date: 2011-12-04
• [Presentation] Molecular biological and epidemiological study of African trypanosomiasis (2011)
  • Author(s): Suzuki T, Ohshima S, Boakye D, Alhassan A, Bosompem K, Ohta N
  • Organizer: SATREPS Joint Workshop
  • Place of Presentation: Accra (Ghana)
  • Year and Date: 2011-12-03