Development of a modified in vivo-expression technology (IVET) usingbacterial outer membrane protein as a reporter, immunoprecipitation,and microarray analysis.

2012 Fiscal Year Final Research Report

• Project/Area Number: 22590391
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bacteriology (including Mycology)
• Research Institution: Osaka University
• Principal Investigator: ABE Hiroyuki 大阪大学, 微生物病研究所, 助教 (00379265)
• Project Period (FY): 2010 – 2012
• Keywords: 百日咳菌 / 気管支敗血症菌 / In vivoexpression technology

Research Abstract

We developed a novel method termed IVET-IP (in vivoexpressed-tag immunoprecipitation system), which enables us to identify bacterial genes expressed in host environments. This method could be useful to search factors that determine the host specificity and pathogenicity of Bordetella spp.

Research Products

• [Journal Article] Bordetella dermonecrotic toxin binds to target cells via the N-terminal 30 amino acids (2011)
  • Author(s): Fukui-Miyazaki A, Ohnishi S, Kamitani S, Abe H, Horiguchi Y
  • Journal Title: Microbiol Immunol. Volume: 55 Pages: 154-159
  • DOI: doi:10.1111/j.13148-0421.2010.00300.x
  • Peer Reviewed
• [Journal Article] Crystal Structure of Clostridium PerfringensEnterotoxin Displays Features of β-Pore-Forming Toxins (2011)
  • Author(s): Kitadokoro, K., K. Nishimura, S. Kamitani, A. Fukui-Miyazaki, H. Toshima, H. Abe, Y. Kamata, Y. Sugita-Konishi, S. Yamamoto, H. Karatani, and Y. Horiguchi
  • Journal Title: J Biol Chem Volume: 286 Pages: 19549-19555
  • DOI: doi:10.1074/jbc.M111.228478
  • Peer Reviewed
• [Journal Article] Enzymatic Actions of Pasteurella MultocidaToxin Detected by Monoclonal Antibodies Recognizing the Deamidated Alpha Subunit of the Heterotrimeric GTPase Gq. (2011)
  • Author(s): Kamitani, S., S. Ao, H. Toshima, T. Tachibana, M. Hashimoto, K. Kitadokoro, A. Fukui-Miyazaki, H. Abe, and Y. Horiguchi
  • Journal Title: FEBS Journal Volume: 278 Pages: 2702-2712
  • DOI: doi:10.1111/j.1742-4658-2011.08197.x
  • Peer Reviewed
• [Journal Article] Characterization of the membrane-targeting C1 domain in Pasteurella multocidatoxin (2010)
  • Author(s): Kamitani S, Kitadokoro K, Miyazawa M, Toshima H, Fukui A, Abe H, Miyake M, Horiguchi Y
  • Journal Title: J Biol Chem Volume: 285 Pages: 25467-25475
  • DOI: doi:10.1074/jbc.M110.102285.
  • Peer Reviewed
• [Presentation] 病原細菌の宿主特異性決定因子の解明 (2012)
  • Author(s): 安倍裕順、神谷重樹、堀口安彦、東秀明
  • Organizer: 人獣共通感染症研究拠点シンポジウム
  • Place of Presentation: 北海道札幌市北海道大学
  • Year and Date: 2012-06-14
• [Presentation] 感染成立過程で発現する病原細菌遺伝子の網羅的解析法の開発 (2012)
  • Author(s): 安倍裕順、神谷重樹、福井理、戸嶋ひろ野、堀口安彦
  • Organizer: 日本細菌学会総会
  • Place of Presentation: 長崎県長崎ブリックホール.
  • Year and Date: 2012-03-27
• [Presentation] IVET- IP法: ラット感染中の気管支敗血症菌の遺伝子発現プロファイル解析. (2012)
  • Author(s): 安倍裕順、神谷重樹、福井理、岡田圭祐、堀口安彦
  • Organizer: 日本細菌学会総会
  • Place of Presentation: 千葉県千葉市幕張メッセ国際会議場.
  • Year and Date: 2012-03-20
• [Presentation] 気管支敗血症菌の新たな病原因子候補. (2012)
  • Author(s): 西川明芳、安倍裕順、岡田圭祐、神谷重樹、福井 理、堀口安彦
  • Organizer: 日本細菌学会総会.
  • Place of Presentation: 千葉県千葉市幕張メッセ国際会議場
  • Year and Date: 2012-03-20
• [Presentation] 感染成立過程で発現する病原細菌遺伝子の網羅的解析法の開発 (2011)
  • Author(s): 安倍裕順、神谷重樹、福井理、戸嶋ひろ野、堀口安彦
  • Place of Presentation: 神奈川県パシフィコ横浜
  • Year and Date: 2011-12-14
• [Presentation] 感染成立過程で発現する病原細菌遺伝子の網羅的解析法の開発 (2011)
  • Author(s): 安倍裕順、神谷重樹、福井理、戸嶋ひろ野、堀口安彦
  • Organizer: 細菌学会関西支部総会
  • Place of Presentation: 大阪府大阪府立大学中百舌鳥キャンパス学術交流会館多目的ホール.
  • Year and Date: 2011-11-19
• [Presentation] 感染成立過程で発現する病原細菌遺伝子の網羅的解析法の開発 (2011)
  • Author(s): 安倍裕順
  • Organizer: 細菌学若手コロッセウム
  • Place of Presentation: 高知県国民宿舎桂浜荘.
  • Year and Date: 2011-08-09
• [Remarks]
  • URL: http://bactox1.biken.osaka-u.ac.jp