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2013 Fiscal Year Final Research Report

Ultra high resolution Xray crystallographic study for antibiotic mechanisms of action and resistance

Research Project

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Project/Area Number 22590401
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionJosai International University

Principal Investigator

NUKAGA Michiyoshi  城西国際大学, 薬学部, 准教授 (20251150)

Co-Investigator(Kenkyū-buntansha) OHUCHI Nozomi  城西国際大学, 環境社会学部, 助教 (20398556)
Co-Investigator(Renkei-kenkyūsha) TANAKA Nobutada  昭和大学, 薬学部, 准教授 (00286866)
HOSHINO Chuji  千葉大学, 薬学研究院, 准教授 (90257220)
BONOMO Robert  Louis Stokes Cleveland Department of Veterans Affairs, オハイオ州、アメリカ合衆国, Professor
Project Period (FY) 2010-04-01 – 2014-03-31
Keywords感染症 / 抗生物質 / 酵素 / 蛋白質 / 構造生物学
Research Abstract

Carbapenems are one of the most important antibiotics and are used worldwide as "Last Resort". We studied four subjects, which are the inhibition mechanisms of carbapenems to beta-lactamases on the basis of Xray crystallography, as listed below. (1) Carbapenems inhibition mechanism for class C beta-lactamases. (2) Crystal structures of the PenA beta-lactamases from Burkholderia cepacia(PenI) and Burkholderia pseudomallei(PenA). (3) Crystal structure of PenA carbapenemase-avibactam complex. (4) Inhibitor screening of class B beta-lactamases.
We are showing the overview of the research with crystal structures.

  • Research Products

    (10 results)

All 2013 2012 2011 2010

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (7 results) (of which Invited: 1 results)

  • [Journal Article] β-ラクタマーゼの構造から考える耐性獲得機構、オキシイミノ系およびカルバペネム系β-ラクタム剤を中心に2013

    • Author(s)
      額賀路嘉
    • Journal Title

      日本化学療法学会雑誌

      Volume: Vol.61 Pages: 479–491

    • Peer Reviewed
  • [Journal Article] In- sights intoβ-lactamases from Burkholderia species, two phylogenetically related yet dis- tinct resistance determinants2013

    • Author(s)
      Papp-Wallace KM, Taracila MA, Gatta JA, Ohuchi N., Bonomo R. A., Nukaga M
    • Journal Title

      Journal of Bi- ological Chemistry

      Volume: Vol.288 Pages: 19090–19102

    • Peer Reviewed
  • [Journal Article] Thrombin-stimulated prolifera- tion is mediated by endothelin-1 in cultured rat gingival fibroblasts2010

    • Author(s)
      Ohuchi N, Hayashi K, Iwamoto K, Koike K, Kizawa Y, Nukaga M, Kakegawa T, Mu- rakami H
    • Journal Title

      Fundam. Clin. Phar- macol

      Volume: Vol.16 Pages: 501–508

    • Peer Reviewed
  • [Presentation] Studies on the Inactivation of Enterobacter cloacae P99 Class C beta- Lactamases by Carbapenems : Doripenem and Panipenem2013

    • Author(s)
      M. Nukaga, N. Ohuchi, C. Bethel, R. A. Bonomo
    • Organizer
      53th ICAAC (Interscience Conference on Antimicrobial Agents and Chemotherapy)
    • Place of Presentation
      デンバー(コロラド州), USA
    • Year and Date
      20130900
  • [Presentation] クラスCβ-ラクタマーゼの構造・反応機構・耐性化メカニズム、シンポジウム;βラクタマーゼ学2012

    • Author(s)
      額賀路嘉
    • Organizer
      第59回日本化学療法学会東日本支部総会
    • Place of Presentation
      お台場、日航ホテル
    • Year and Date
      20121000
    • Invited
  • [Presentation] Crystal Structures of the Pen beta-Lactamases of Burkholde- ria cepacia (Bc) and Burkholderia pseudo- mallei (Bp)2012

    • Author(s)
      K. M. Papp-Wallace, R. A. Bonomo, N. Ohuchi, M. Nukaga
    • Organizer
      52th Interscience Conference on Antimicrobial Agents and Chemother- apy(ICAAC), Sept
    • Place of Presentation
      サンフランシスコ, USA
    • Year and Date
      20120000
  • [Presentation] The Contribution of Ambler Po- sition P167 in Ceftazidime(TAZ) Resistance in PenI of Burkholderia pseudomallei (Bp)2012

    • Author(s)
      K. M. Papp-Wallace, M. Nukaga, R. A. Bonomo
    • Organizer
      52th Interscience Conference on Antimi- crobial Agents and Chemotherapy(ICAAC), Sept
    • Place of Presentation
      サンフランシスコ, USA
    • Year and Date
      20120000
  • [Presentation] Structure-function studies of PenAβ-lactamase : insights into single amino acid substitutions that mediate ceftazidime (TAZ) resistance in Burkholderia pseudomallei (Bp)2011

    • Author(s)
      K. M. Papp-Wallace, M. Nukaga, D.A. Rholl, H. P. Schweizer, M. Taracila, R. A. Bonomo
    • Organizer
      51th Interscience Conference on Antimicro- bial Agents and Chemotherapy (ICAAC) Sept 17–20
    • Place of Presentation
      Chicago, IL, USA
    • Year and Date
      20110000
  • [Presentation] Studies on the Inactiva- tion of Class C beta-Lactamases by Aztre- onam (ATM)2011

    • Author(s)
      M. Nukaga, C. R. Bethel, S. M. Drawz, R. A. Bonomo
    • Organizer
      51th Interscience Conference on Antimicrobial Agents and Chemotherapy (ICAAC) Sept 17–20
    • Place of Presentation
      Chicago, IL, USA
    • Year and Date
      20110000
  • [Presentation] Studies on the Inactivation of Class C beta-Lactamases by Carbapen- ems : Panipenem and P992010

    • Author(s)
      M. Nukaga, C. R. Bethel, S. Drawz, R. A. Bonomo
    • Organizer
      50th Inter- science Conference on Antimicrobial Agents and Chemotherapy (ICAAC), Sept. 12–15
    • Place of Presentation
      Boston, MA, USA
    • Year and Date
      20100000

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Published: 2015-07-16  

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