Identification of the botulinum toxin receptor protein on the intestinal epithelial cells

2012 Fiscal Year Final Research Report

• Project/Area Number: 22590405
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bacteriology (including Mycology)
• Research Institution: Tokyo University of Agriculture
• Principal Investigator: WATANABE Toshihiro 東京農業大学, 生物産業学部, 教授 (80175695)
• Co-Investigator(Renkei-kenkyūsha): OOYAMA Toru 東京農業大学, 生物産業学部, 教授 (60318178) ; NIWA Koichi 東京農業大学, 生物産業学部, 教授 (20301012)
• Project Period (FY): 2010 – 2012
• Keywords: 細菌学(含真菌学)

Research Abstract

Botulinum toxin complex (TC) is composed of neurotoxin, NTNHA,HA-70, HA-33, and HA-17. TC enters into blood stream via small intestinal epithelium. In this study, we revealed that the toxin complex binds to the different sugar-chain depending on the struc

Research Products

• [Journal Article] Characterization of sugar recognition by the toxin complex produced by the Clostridium botulinum serotype C variant strain Yoichi (2011)
  • Author(s): Matsuo, T., Miyata, K. Niwa, K. Watanabe, T. Ohyama, T
  • Journal Title: FEMS Immunol. Med. Microbiol Volume: 63(1) Pages: 35-43
  • DOI: doi:10.1111/j.1574-695X.2011.00825.x
  • Peer Reviewed
• [Journal Article] HA-33 facilitates transport of the serotype D botulinum toxin across a rat intestinal epithelial cell monolayer (2011)
  • Author(s): Ito, H., Sagane, Y. Niwa, K. Ohyama, T. Ohyama, T
  • Journal Title: FEMS Immunol. Med. Microbiol Volume: 61(3) Pages: 323-331
  • DOI: doi:10.1111/j.1574-695X.2011.00779.x
  • Peer Reviewed
• [Presentation] ボツリヌス神経毒素結合タンパク質 非毒非血球凝集素の小腸上皮細胞への結合および透過機構 (2013)
  • Author(s): 宮下慎一郎,相根義昌,犬井健,栗原小百合,鈴木智典,丹羽光一,渡部俊弘
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 千葉
  • Year and Date: 20130318-20
• [Presentation] ボツリヌス神経毒素結合タンパク質-非毒非血球凝集素の小腸上皮細胞層への結合および透過機構 (2012)
  • Author(s): 宮下慎一郎,相根義昌,犬井健,栗原小百合,狩野聡史,林慎太郎,川根武紘,鈴木智典,丹羽光一,渡部 俊弘
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
  • Year and Date: 20121214-16
• [Presentation] Transport of the botulinum neurotoxin-associating protein, nontoxic nonhemagglutinin, across the intestinal epithelial cell layer (2012)
  • Author(s): Miyashita, S. -I., Sagane, Y., Miyata, K., Inui, K., Kurihara, S., Kano, S., Hayashi, S., Kawane, T., Suzuki, T., Niwa, K., and Watanabe, T
  • Organizer: 22nd IUBMB and 37th FEBS
  • Place of Presentation: Seville, Spain
  • Year and Date: 20120904-09
• [Presentation] ボツリヌス神経毒素結合タンパク質非毒非血球凝集素の小腸上皮細胞への結合および透過 (2012)
  • Author(s): 宮下慎一郎,相根義昌,宮田恵多,犬井健,栗原小百合,狩野聡史,林慎太郎,川根武紘,鈴木智典,丹羽光一,渡部俊弘
  • Organizer: 第79回日本細菌学会北海道支部会学術総会
  • Place of Presentation: 帯広
  • Year and Date: 20120828-29
• [Presentation] C 型ボツリヌス毒素の小腸上皮細胞からの侵入機構の解析 (2010)
  • Author(s): 犬井 健,宮田恵多,伊藤博章,松尾 5,堀内亮太,吉田悠真,宮下慎一郎,村松将司,渡部俊弘,大山 徹,丹羽光一
  • Organizer: 第33回日本分子生物学会年会代 83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 20101207-10
• [Presentation] C 型およびD 型ボツリヌス毒素の小腸上皮細胞の透過におけるシアル酸の役割 (2010)
  • Author(s): 丹羽光一,伊藤博章,犬井 健,宮田恵多,松尾智仁,堀内亮太,宮下慎一郎,吉田悠真,村松将司,渡部俊弘,大山 徹
  • Organizer: 第150回日本獣医学会学術集会
  • Place of Presentation: 帯広
  • Year and Date: 20100916-18
• [Book] Structure and function of food engineering. Ayman Amer Eissa (2012)
  • Author(s): Sagane, Y., Inui, K., Miyashita, S. -I., Miyata, K., Suzuki, T., Niwa, K., and Watanabe, T
  • Total Pages: 137-150
  • Publisher: Botulinum toxin complex: a delivery vehicle of botulinum neurotoxin traveling digestive tract
• [Patent(Industrial Property Rights)] http://www.bioindustry.nodai.ac.jp/~seika/
  • Inventor(s): 生物化学研究室ホームページ