2012 Fiscal Year Final Research Report

Characterization of immunotoxicological and immunopathological roles of fungal PAMPs

Research Project

Project/Area Number	22590406
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Bacteriology (including Mycology)
Research Institution	Tokyo University of Pharmacy and Life Science
Principal Investigator	OHNO Naohito 東京薬科大学, 薬学部, 教授 (80152213)
Co-Investigator(Renkei-kenkyūsha)	ADACHI Yoshiyuki 東京薬科大学, 薬学部, 准教授 (60222634) MIURA Noriko 東京薬科大学, 薬学部, 講師 (60222634) ISHIBASHI Ken-ichi 東京薬科大学, 薬学部, 助教 (60222634)
Project Period (FY)	2010 – 2012
Keywords	感染免疫 / PAMPs
Research Abstract	DBA/2 is a strain, specifically higher response to fungal PAMPs, beta-glucan. In the present study, immunotoxicological and immunopathological rolesof fungal PAMPs were examined. From the results of this study, we have demonstratedthat DBA/2 mice is a highly sensitive strain to produce high level of inflammatory cytokinein vitro and in vito. In vitro study, DBA/2 mice produced significantly high level of inflammatory cytokines, such as GM-CSF, IFN-gamma, and TNF-alpha. It is highly sensitive tothe cross-linking of cytoskeleton and modulated by disrupting actin polymerization. In vivo study, producing high concentration of anti-beta-glucan antibody and induce lethal toxicity. From these findings, it is a good tool to analyze beta-glucan mediated immunotoxicological response.

(5 results)