2012 Fiscal Year Final Research Report
Examination of rapid and easy detection methods for fungal beta-glucans
| Project/Area Number |
22590407
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Tokyo University of Pharmacy and Life Science |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
OHNO Naohito 東京薬科大学, 薬学部, 教授 (80152213)
MIURA Noriko 東京薬科大学, 薬学部, 講師 (30218036)
ISHIBASHI Ken-ichi 東京薬科大学, 薬学部, 助教 (20453805)
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| Project Period (FY) |
2010 – 2012
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| Keywords | ss-グルカン / 深在性真菌症 / ss-グルカン認識タンパク / ELISA / イムノクロマトグラフィー |
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| Research Abstract |
To develop a specific detection methods for fungal products, particularly beta-glucans found in blood of mycosis patient, we examined various beta-glucan recognition proteins (BGRP) from invertebrates and mammalian. The binding specificities of the recombinant BGRPs to the beta-glucans from various natural source, including fungi, bacterium, algae, and plants were compared to choose suitable protein probes for the detection. The combination of selected BGRPs was applied for ELISA methods and immunochromatography. Using those devices, we could detect trace level of beta-glucans in the blood from animal model of fungal infection.
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