Role of tumor-suppressive genes on LPS-induced inflammatory response

2012 Fiscal Year Final Research Report

• Project/Area Number: 22590408
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bacteriology (including Mycology)
• Research Institution: Aichi Medical University
• Principal Investigator: YOKOCHI Takashi 愛知医科大学, 医学部, 教授 (20126915)
• Project Period (FY): 2010 – 2012
• Keywords: LPS / ASAP1 / RAW264.7 NF-kappaB / 炎症性メディエーター

Research Abstract

The involvement of retinoblastoma protein-interacting zinc finger 1 (RIZ1), a tumor suppressor, in lipopolysaccharide (LPS)-induced inflammatory responses was investigated by using RAW 264.7 macrophage-like cells. RIZ1 was suggested to augment LPS-induced NF-kappaB activation in collaboration with p53 and enhance the production of proinflammatory cytokines in response to LPS. An ADP ribosylation factor-GTPase activating protein (ASAP1) is constitutively expressed in the cells and the expression was augmented by LPS stimulation. Silencing of ASAP1 enhanced the production of proinflammatory mediators in response to LPS. A series of tumor-associated genes are involved in LPS-induced inflammatory response in macrophages.

Research Products

• [Journal Article] An ADP ribosylationfactor-GTPase activating protein negatively regulates the production of proinflammatory mediators in response to lipopolysaccharide. (2011)
  • Author(s): Haque A, Noman AS, Koide N, Odkhuu E, Naiki Y, Hashimoto S, Komatsu T, Yoshida T, Yokochi T.
  • Journal Title: Cancer Immunol Immunother. Volume: 60 Pages: 1439-46
  • DOI: doi:10.1007/s00262-011-1048-9.
• [Journal Article] Retinoblastoma protein-interacting zinc finger 1 (RIZ1) participates in RANKL-induced osteoclast formation via regulation of NFATc1expression. (2010)
  • Author(s): Noman AS, Koide N, Iftakhar-E-Khuda I, Dagvadorj J, Tumurkhuu G, Naiki Y, Komatsu T, Yoshida T, Yokochi T.
  • Journal Title: Immunol Lett. Volume: 131 Pages: 166-9
  • DOI: doi:10.1016/j.imlet.2010.04.006.
• [Journal Article] Seladin-1 is a novel lipopolysaccharide (LPS)-responsive geneand inhibits the tumour necrosis factor-alpha production and osteoclast formation in response to LPS. (2010)
  • Author(s): Khuda II, Koide N, Noman AS, Dagvadorj J, Tumurkhuu G, Naiki Y, Komatsu T, Yoshida T, Yokochi T.
  • Journal Title: Immunology Volume: 131 Pages: 59-66
  • DOI: doi:10.1111/j.1365-2567.2010.03274.x.
• [Journal Article] Retinoblastoma protein-interacting zinc finger 1, a tumor suppressor, augments lipopolysaccharide-induced proinflammatory cytokine production via enhancing nuclear factor-kappaB activation. (2010)
  • Author(s): Noman AS, Koide N, Iftakhar-E-Khuda I, Dagvadorj J, Tumurkhuu G, Naiki Y, KomatsuT, Yoshida T, Yokochi T.
  • Journal Title: Cell Immunol Volume: 264 Pages: 114-8
  • DOI: doi:10.1016/j.cellimm.2010.05.007.
• [Journal Article] Retinoblastoma protein-interacting zinc finger 1 (RIZ1)regulates the proliferation of monocytic leukemia cells via activation of p53. (2010)
  • Author(s): Shadat NM, Koide N, Khuda II, Dagvadorj J, Tumurkhuu G, Naiki Y, Komatsu T, Yoshida T, Yokochi T.
  • Journal Title: Cancer Invest. Volume: 28 Pages: 806-12
  • DOI: doi:10.3109/07357907.2010.494323.
• [Presentation] on lipopolysaccharide-induced nitric oxide via reducing interferon-ss production.The novel inhibitory effect of pifithrin (PFT)-α, an inhibitor of p53 (2012)
  • Author(s): Erdenezaya Odkhuu et al.
  • Organizer: 第12回国際エンドトキシン自然免疫学会
  • Place of Presentation: 東京
  • Year and Date: 2012-10-24
• [Presentation] Lipopolysaccharide prevents valproicacid-induced apoptosis via activation of NF-κB andinhibition of p53 activation. (2012)
  • Author(s): Bilegtsaikhan Tsolmongyn et al.
  • Organizer: 第12回国際エンドトキシン自然免疫学会
  • Place of Presentation: 東京
  • Year and Date: 2012-10-24