2012 Fiscal Year Final Research Report
Pathophysiologial role of Helicobacter pylori asparaginase
| Project/Area Number |
22590410
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
SHIBAYAMA Keigo 国立感染症研究所, 細菌第二部, 部長 (50283437)
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| Project Period (FY) |
2010 – 2012
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| Keywords | ヘリコバクターピロリ / アスパラギナーゼ / 代謝酵素 / 病原性 / 感染成立 / アスパラギン取り込み / 免疫回避 / 胃がん |
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| Research Abstract |
Helicobacter pylori sparaginase exhibited striking cytotoxic activity against U937 cells via asparagine deprivation. The cytotoxic activity of live H. pylori cells against U937 cells was significantly diminished by deletion of the asparaginase gene. An asparaginase-deficient mutant strain was significantly less capable of colonizing Mongolian gerbils. H. pylori cells incorporated extracellular asparagine by transporting aspartate produced by the action of the asparaginase. H. pylori asparaginase may be involved in inhibition of normal lymphocyte function at the gastric niche, allowing H. pylori to evade the host immune system.
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