Influence of HIV protein kinetics on anti-HIV effects in HIV-specific cellular immune response

2012 Fiscal Year Final Research Report

• Project/Area Number: 22590412
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Virology
• Research Institution: The University of Tokyo
• Principal Investigator: TACHIKAWA Ai 東京大学, 医科学研究所, 准教授 (10396880)
• Co-Investigator(Kenkyū-buntansha): MIURA Toshiyuki 長崎大学, 熱帯医学研究所, 客員教授 (00296576)
• Project Period (FY): 2010 – 2012
• Keywords: 病原性 / HIV 感染症 / CTL / 抗原提示 / エスケープ変異

Research Abstract

Strong and broad HIV-specific CTLs are induced against numerous targets (epitopes) during HIV infection. In this study, we demonstrates a mechanism, which generates de novo CTL epitope after one amino acid change that might result from the CTL pressure to the overlapping epitope. This novel “epitope switching” mechanism that occurs in host immunity might help the understanding of cellular immunity and CTL vaccine development.

Research Products

• [Journal Article] Generation of rejuvenated antigen-specific T cells by pluripotency reprogramming and redifferentiation (2013)
  • Author(s): Nishimura T, Kaneko S, Kawana-Tachikawa A, Tajima Y, Gotoh H, Zhu D, Nakayama K, Iriguchi S, Uemura Y, Shimizu T, Takayama N, Yamada D, Nishimura K, Ohtaka M, Watanabe N, Takahashi S, Iwamoto A, Koseki H, Nakanishi M, Eto K, Nakauchi H.
  • Journal Title: Cell Stem Cell Volume: 12 Pages: 114-26
  • Peer Reviewed
• [Journal Article] Significant Reductions in Gag-protease MediatedHIV-1 Replication Capacity Over theCourse of the Epidemic (2013)
  • Author(s): Nomura S, Hosoya N, Brumme ZL, Brockman MA, Kikuchi T, Koga M, Nakamura H, Koibuchi T, Fujii T, Carlson JM, Heckerman D, Kawana-Tachikawa A, Iwamoto A, Miura T.
  • Journal Title: Japan. J Virol Volume: 87 Pages: 1465-76
  • DOI: doi:10.1128/JVI.02122-12
  • Peer Reviewed
• [Journal Article] Improved neutralizing antibody response in the second season after a single dose of pandemic (H1N1) 2009 influenza vaccine in HIV-1-positive adults (2012)
  • Author(s): Kikuchi T, Iwatsuki-Horimoto K, Adachi E, Koga M, Nakamura H, Hosoya N, Kawana-Tachikawa A, Koibuchi T, Miura T, Fujii T, Kawaoka Y, Iwamoto A.
  • Journal Title: Vaccine Volume: 30 Pages: 3819-23
  • DOI: doi:10.1016/j.vaccine.2012.03.083
  • Peer Reviewed
• [Journal Article] Imbalanced production of cytokines by T cells associates with the activation/exhaustion status of memory T cells in chronic HIV Type 1 infection (2012)
  • Author(s): Nakayama K, Nakamura H, Koga M, Koibuchi T, Fujii T, Miura T, Iwamoto A, Kawana-Tachikawa A.
  • Journal Title: AIDS Res Hum Retroviruses Volume: 28 Pages: 702-14
  • DOI: doi:10.1089/AID.2011.0073
  • Peer Reviewed
• [Journal Article] Long-term successful control of super-multidrug-resistant human immunodeficiency virus type 1 infection by a novel combination therapy of raltegravir,etravirine, and boosted-darunavir (2011)
  • Author(s): Nakamura H, Miyazaki N, Hosoya N, Koga M, Odawara T, Kikuchi T, Koibuchi T, Kawana-Tachikawa A, Fujii T, Miura T, Iwamoto A.
  • Journal Title: J Infect Chemother Volume: 17 Pages: 105-10
  • Peer Reviewed
• [Journal Article] HIV-1 tropism (2010)
  • Author(s): Iwamoto A, Hosoya N, Kawana-TachikawaA.
  • Journal Title: Protein Cell Volume: 1 Pages: 510-3
  • DOI: doi:10.1007/s13238-010-0066-2
  • Peer Reviewed
• [Journal Article] Novel immunodominant peptide presentation strategy: a featured HLA-A*2402 restricted CTL-epitope stabilized by intra-chain hudrogen-bonds from SARS-CoV nucleocapsid protein (2010)
  • Author(s): Liu J, Wu P, Gao F, Qi J, Kawana-Tachikawa A, Xie J, Vavricka CJ, Iwamoto A, Li T, Gao GF.
  • Journal Title: J Virol Volume: 84 Pages: 11849-57
  • DOI: doi:10.1128/JVI.01464-10
  • Peer Reviewed
• [Journal Article] Changes in impact of HLA class I allele expression on HIV-1 plasma virus loads at a population level over time (2010)
  • Author(s): Koga M, Kawana-Tachikawa A, Heckerman D, Odawara T, Nakamura H, Koibuhi T, Fujii T, Miura T, Iwamoto A.
  • Journal Title: MicrobiolImmunol Volume: 54 Pages: 196-205
  • DOI: doi:10.1111/j.1348-0421.2010.00206.x
  • Peer Reviewed
• [Presentation] Prolonged preservation of HIV-specific cellular immunity in recently HIV-infected individuals receiving Maraviroc intensified HAART. (2013)
  • Author(s): Kawana-Tachikawa A, Llibre JM, Bravo I, Escrig R, Mothe B, Miro JM, Gatell JM, Iwamoto A, Clotet B, Brander C.
  • Organizer: 20th conference on retroviruses and opportunistic infections
  • Place of Presentation: Atlanta, USA
  • Year and Date: 20130300
• [Presentation] Impact of an amino acid change within overlapping CTL epiotpes in HIV-1 infection. Keystone symposia (2013)
  • Author(s): Han Chungyong, Shimizu A, Zhu D, Kawana-Tachikawa A, Iwamoto A.
  • Organizer: HIV Vaccines
  • Place of Presentation: Keystone Colorado, USA
  • Year and Date: 20130200
• [Presentation] Mechanisms underlying disruption of T cell immunity during chronic HIV-1 infection. The 2nd Joint Global COE Symposium of the IMSUT & RCAST Global COE, The University of Tokyo and Chiba University Global COE "Current and Future Trends in Genome-based Immunity (2013)
  • Author(s): Kawana-Tachikawa A.
  • Organizer: Infection and Cancer
  • Place of Presentation: Tokyo
  • Year and Date: 20130100
• [Presentation] HIV-1elitecontrollerにおけるHIV-1Vifの抗APOBEC3G活性の低下 (2012)
  • Author(s): 菊地正、岩部幸枝、立川(川名)愛、古賀道子、野村滋、細谷紀彰、BrummeZL,HeikoJ,KelleherAD,MarkowitzM,PereyraF,TrochaA,WalkerBD,岩本愛吉、徳永研三、三浦聡之.
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 20121100
• [Presentation] 感染早期でのHAART 開始時における MaravirocによるHAART 増強の T細胞に与える影響 (2012)
  • Author(s): 立 川(川名)愛 、Beatriz Mothe-Pujadas, Marta Massanella, Josep M Libre, Bonaventura Clotet, 岩 本 愛 吉 , Christian Brander.
  • Organizer: 第26回日本エイズ学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 20121100
• [Presentation] Anti-APOBEC3G activity of HIV-1 Vifprotein from elite controllers is attenuated compared to those from untreated chronic progressors of those from individuals with acute infection. (2012)
  • Author(s): Kikuchi T, Iwabu Y, Kawana-Tachikawa A, Koga M, Nomura S, Hosoya N, Brumme ZL, Heiko J, Kelleher AD, Markowitz M, Pereyra F, Trocha A, Walker BD, Iwamoto A, Tokunaga K, Miura T.
  • Organizer: XIX International AIDS Conference
  • Place of Presentation: Washington DC, USA.
  • Year and Date: 20120700
• [Presentation] HIV感染者におけるパンデミック2009(H1N1)インフルエンザワクチンの初回接種前後と2年目の接種前後の中和抗体価の推移. (2012)
  • Author(s): 菊地正、岩附研子、安達英輔、古賀道子、中村仁美、立川愛、鯉渕智彦、藤井毅、河岡義裕、岩本愛吉.
  • Organizer: 第12回東京大学生命科学シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 20120600
• [Presentation] Development of a HIV-1 phenotypic tropism assay using dual split protein (DSP)-mediated membrane fusion detection system". (2012)
  • Author(s): Hosoya N. Teeranaipong P,Kawana-Tachikawa A, Kondo N, Hoshino H, Matsuda Z, Iwamoto A.
  • Organizer: 1st Asian Conference on Hepatitis B and C, HIV and Influenza
  • Place of Presentation: Beijing, China
  • Year and Date: 20120500
• [Presentation] HIV感染慢性期におけるT細胞の免疫病態 (2011)
  • Author(s): 立川(川名)愛
  • Organizer: 第25回日本エイズ学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 20111200
• [Presentation] 無症候慢性HIV-1陽性者由来gag-proteaseを発現するキメラNL4-3ウイルス複製能の患者初診年による変化. (2011)
  • Author(s): 野村滋,菊地正,細谷紀彰,古賀道子,中村仁美,鯉渕智彦,藤井毅,立川愛,岩本愛吉,三浦聡之
  • Organizer: 第25回日本エイズ学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 20111200
• [Presentation] HIV-1サブタイプ未同定の検体を用いた配列特異的オリゴプローブによる薬剤耐性変異の検出. (2011)
  • Author(s): 谷麗君、細谷紀彰、立川(川名)愛、中村仁美、古賀道子、松下正毅、BiyanLu、三浦聡之、岩本愛吉.
  • Organizer: 第25回日本エイズ学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 20111200
• [Presentation] Replication capacities of chimeric NL4-3 encoding gag-protease from modern HIV-1 isolates are significantly reduced compared to those derived from isolates in the early days of epidemic in Japan (2011)
  • Author(s): Nomura S, Hosoya N, Kikuchi T, Koga M, Nakamura H, Koibuchi T, Fujii T, Kawana-Tachikawa A, Iwamoto A, Miura T.
  • Organizer: 6th International AIDS Society Conference on HIV Pathogenesis, Treatment and Prevention
  • Place of Presentation: Rome Italy
  • Year and Date: 20110700
• [Presentation] Induction and maintenance of neutralizing antibodies after single dose of non-adjuvanted split 2009 pandemic H1N1 influenza A vaccine in HIV-1 positive population. (2011)
  • Author(s): Kikuchi T, Iwatsuki-Horimoto K, Adachi E, Imai K, Shimizu S, Miyazaki N, Koga M, Nakamura H, Kawana-Tachikawa A, Koibuchi T, Miura T, Fujii T, Kawaoka Y, Iwamoto A.
  • Organizer: 6th International AIDS Society Conference on HIV Pathogenesis, Treatment and Prevention
  • Place of Presentation: Rome Italy
  • Year and Date: 20110700
• [Presentation] Development of a PCR-SSOP-Liminex Assay for HIV-1 Drug Resistance. Session IV: Virology, Novel Therapies, and New Technologies. (2010)
  • Author(s): Gu L, Kawana-Tachikawa A, Shiino T, Hosoya N, Nunoya J, Koibuchi T, Fujii T, Miura T, Matsushita M, Sugiura W, and Iwamoto A.
  • Organizer: 24th Joint Meeting of the AIDS Panels, United States-Japan Cooperative Medical Science Program. HIV Resistance
  • Place of Presentation: Asia. Grand Copthorne Hotel, Singapore
  • Year and Date: 20101200
• [Presentation] Novel HIV-1 Phenotypic Tropism Assay without Psuedotyoed Virions. Session IV: Virology, Novel Therapies, and New Technologies.24th Joint Meeting of the AIDS Panels,United States-Japan Cooperative Medical Science Program (2010)
  • Author(s): Teeranaipong P, Hosoya N, Kawana-Tachikawa A. Kondo N, Hoshino H, Matsuda Z, and Iwamoto A.
  • Organizer: HIV Resistance
  • Place of Presentation: Asia. Grand Copthorne Hotel, Sigapore
  • Year and Date: 20101200
• [Presentation] T ell activation and exhaustion associates with skewed cytokines/chemokines production in chronic HIV-1 infection. (2010)
  • Author(s): Nakayama K, Kawana-Tachikawa A, Nakamura H, Miura T, Fujii T, Koibuchi T, Iwamoto A.
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: Kobe, Japan
  • Year and Date: 20100800
• [Presentation] HIV-1 viral burden has an impact on Th1- and Th17-related cytokines/chemokines production of T cells. (2010)
  • Author(s): Nakayama K, Kawana-Tachikawa A, Nakamura H, Miura T, Fujii T, Koibuchi T, Iwamoto A.
  • Organizer: 18th International AIDS Conference
  • Place of Presentation: Vienna, Austria
  • Year and Date: 20100700
• [Remarks]
  • URL: http://www.idimsut.jp/