2012 Fiscal Year Final Research Report
Analysis of the role of Japanese encephalitis virus NS4A protein in its pathogenicity
| Project/Area Number |
22590423
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
TAJIMA Shigeru 国立感染症研究所, ウイルス第一部, 主任研究官 (60311346)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 日本脳炎ウイルス / NS4A / 病原性 |
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| Research Abstract |
In this study we showed that 1) Japanese encephalitis virus (JEV)with a 3-Ile residue in NS4A protein exhibits higher neuroinvasiveness than JEV with 3-Val in the protein, 2) approximately 20% of the JEV isolates in Japan have 3-Ile residue in NS4A, and in the 3-Ile group we found a strain with significantly weaker virulence as compared with the other 3-Ile strains, 3) a Ser-to-Asn substitution at position 123 in the JEV E protein has weak impact on viral growth properties in vitro or on pathogenicity in vivo, 4) the short VR in JEV 3’ non-translated region do not affect its growth in vitro or its pathogenicity in mice.
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[Journal Article] serine-to-asparagine substitution at position 123 in the Japanese encephalitis virus E protein.2013
Author(s)
Yamaguchi, Y., Nukui, Y., Kotaki, A., Sawabe, K., Saijyo, M., Watanabe, H., Kurane, I., Takasaki, T., and Tajima, S
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Journal Title
Journal of General Virology
Volume: 94
Pages: 90-96
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[Journal Article] Identification and characterization of the short variable region of the Japanese encephalitis virus 3' NTR.2012
Author(s)
2)Kato, F., Kotaki, A., Yamaguchi, Y., Shiba, H., Hosono, K., Harada, S., Saijo, M, Kurane, I., Takasaki, T., and Tajima, S
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Journal Title
Virus Genes
Volume: 44
Pages: 191-197
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[Journal Article] An amino acid substitution (V3I) in the Japanese encephalitis virus NS4A protein increases its virulence in mice, but not its growth rate in vitro.2011
Author(s)
Yamaguchi, Y., Nukui, Y., Tajima, S., Nerome, R., Kato, F., Watanabe, H., Takasaki, T., and Kurane, I.
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Journal Title
Journal of General Virology
Volume: 92
Pages: 1601-1606
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