2012 Fiscal Year Final Research Report
Study on cytomegalovirus: cell tropism, pathogenesis, and induction of protective antibodies.
| Project/Area Number |
22590426
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
INOUE Naoki 国立感染症研究所, ウイルス第一部, 室長 (90183186)
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| Research Collaborator |
HASHIMOTO Kaede 国立感染症研究所, ウイルス第一部, 研究生
FUKUCHI Saki 国立感染症研究所, ウイルス第一部, 研究生
FUKUI Yoshiko 国立感染症研究所, ウイルス第一部, 非常勤職員
TSUDA Mihoko 国立感染症研究所, ウイルス第一部, 非常勤職員
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| Project Period (FY) |
2010 – 2012
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| Keywords | サイトメガロウイルス / モルモット / 感染防御 / 糖蛋白 / 細胞指向性 |
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| Research Abstract |
In contrast to murine and rat CMVs, guinea pig CMV (GPCMV) crosses the placenta and causes infection in utero, which makes GPCMV animal models useful for studies on the mechanisms of transplacental transmission of CMV. Previously, we reported that the GPCMV stock purchased from the ATCC contained two types of strains, one containing and the other lacking a 1.6 kb locus that encodes human CMV UL128 and UL130 homologs, GP129 and GP131, and that the 1.6 kb locus was required for efficient viral growth in animals but not in cell culture. HCMV UL128/130/131A form a pentamer complex with gH/gL and play a role in the endothelial/epithelial-tropism. In this study, we constructed a BAC containing a GPCMV genome, the virus from which grew normally in guinea pig fibroblast cells GPL. Using the RedET recombination system for BAC, mutations were introduced into each of GP129, GP131, and GP133 ORFs. Although all mutants grew in GPL and epithelial cell line GPC-16 at the level similar to a virus derived from the BAC with the wild-type sequence, they could not infect monocytes/ macrophages. GPCMV was transmittable to fibroblast from infected macrophages by co-culturing. Since macrophages play an important role in dissemination of virus to the organs, the macrophage tropism may associate with efficient viral growth in vivo.
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[Journal Article] The lack of antibodies against the AD2 epitope of cytomegalovirus (CMV) glycol- protein B (gB) is associated with CMV disease after renal transplantation in recipients having gH serotypes same as their donors.2011
Author(s)
Ishibashi K, Tokumoto T, Shirakawa H, Hashimoto K, Ikuta K, Kushida N, Yanagida T, Shishido K, Aikawa K, Toma H, Inoue N, Yamaguchi O, Tanabe K, Suzutani T
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Journal Title
Transplant. Infect. Dis.
Volume: 13
Pages: 318- 28
DOI
Peer Reviewed
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[Presentation] A genotypic and serologic study of cytomegalovirus (CMV) reinfection in mothers and neonates with congenital CMV infection in Japan.2011
Author(s)
Kazufumi Ikuta, Ken Ishioka, Takashi Imamura, Kimisato Asano, Tetsushi Yoshikawa, Hiroyuki Moriuchi, Shigeyoshi Fujiwara, Takahiko Kubo, Shin Koyano, Naoki Inoue, Tatsuo Suzutani
Organizer
XV International Congress of Virology
Place of Presentation
Sapporo
Year and Date
20110900
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