2012 Fiscal Year Final Research Report
Analysis of native immune responses to Epstein-Barr virus in the virus-infected humanized mice
| Project/Area Number |
22590430
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | National Research Institute for Child Health and Development |
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Principal Investigator |
FUJIWARA Shigeyoshi 独立行政法人国立成育医療研究センター, 母児感染研究部, 部長 (30173488)
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| Co-Investigator(Kenkyū-buntansha) |
IMADOME Ken-ichi 国立成育医療研究センター, 母児感染研究部, 室長 (70392488)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 感染防御 / ワクチン |
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| Research Abstract |
Splenic NK cells isolated from EBV-infected humanized mice two days post-infection and cultured for two days produced higher levels of IFN_<-γ> as compared with the same cells isolated from uninfected humanized mice and processed similarly. Activated T-cell preparations were generated from cord blood by culturing with IL-2 and anti-CD3 antibody. When this activated T-cell preparation was administered to EBV-infected humanized mice generated with hematopoietic stem cells isolated from the same cord blood sample, therapeutic effects was evident as decreased EBV DNA levels in mouse organs and prolonged life span.
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Research Products
(20 results)
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[Journal Article] Effective control of Epstein-Barr virus infection following pediatric liver transplantation by monitoring of viral DNA load and lymphocyte surface markers.2012
Author(s)
Imadome K, Fukuda A, Kawano F, Imai Y, Ichikawa S, Mochizuki M, Shigeta T, Kakiuchi T, Sakamoto S, Kasahara M, Fujiwara S
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Journal Title
Pediatr Transplant.
Volume: 16(7)
Pages: 748-57
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[Journal Article] ZNF385B is characteristically expressed in germinal center B cells and involved in B-cell apoptosis.2012
Author(s)
Iijima K, Yamada H, Miharu M, Imadome K, Miyagawa Y, Akimoto S, Kobayashi K, Okita H, Nakazawa A, Fujiwara S, Fujimoto J, Kiyokawa N.
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Journal Title
Eur J Immunol.
Volume: 42(12)
Pages: 3405-15
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[Journal Article] Characterization of Epstein-Barr virus (EBV)-infected cells in EBV-associated hemophagocytic lymphohistiocytosis in two patients with X-linked lymphoproliferative syndrome type 1 and type 2.2012
Author(s)
Yang X, Wada T, Imadome K, Nishida N, Mukai T, Fujiwara M, Kawashima H, Kato F, Fujiwara S, Yachie A, Zhao X, Miyawaki T, Kanegane H
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Journal Title
Herpesviridae
Volume: 3
Pages: 1
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[Journal Article] Novel Mouse Xenograft Models Reveal a Critical Role of CD4+ T Cells in the Proliferation of EBV-Infected T and NK Cells.2011
Author(s)
Imadome K, Yajima M, Arai A, Nakazawa A, Kawano F, Ichikawa S, Shimizu N, Yamamoto N, Morio T, Ohga S, Nakamura H, Ito M, Miura O, Komano J, and Fujiwara S
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Journal Title
PLoS Pathogens
Volume: 7(10)
Pages: e1002326
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[Journal Article] Epstein-Barr Virus Induces Erosive Arthritis in Humanized Mice.2011
Author(s)
Kuwana Y, Takei M, Yajima M, Imadome K, Inomata H, Shiozaki M, Ikumi N, Nozaki T, Shiraiwa H, Kitamura N, Takeuchi J, Sawada S, Yamamoto N, Shimizu N, Ito M, and Fujiwara S
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Journal Title
PLoS ONE
Volume: 6(10)
Pages: e26630
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[Journal Article] Bortezomib Induces Apoptosis in T Lymphoma Cells and Natural Killer Lymphoma Cells Independent of Epstein-Barr Virus Infection.2011
Author(s)
Iwata S, Yano S, Ito Y, Ushijima Y, Gotoh K, Kawada J, Fujiwara S, Sugimoto K, Isobe Y, Nishiyama Y, Kimura H
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Journal Title
Int J Cancer
Volume: 129
Pages: 2263-2273
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