Application of rapid gene analyzing method for susceptibility to cancer

2012 Fiscal Year Final Research Report

• Project/Area Number: 22590516
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Gunma University
• Principal Investigator: SHIMIZU Kimihiro 群馬大学, 医学部, 講師 (90375535)
• Co-Investigator(Kenkyū-buntansha): ALEXANDER LEZHAVA 独立行政法人理化学研究所, 上級研究員 (40443048) ; USUI KENZGO 独立行政法人理化学研究所, 研究員 (10525372) ; SUNAGA NORIAKI 群馬大学, 大学院・医学系研究科, 助教 (70400778) ; TANAKA KAZUMI 群馬大学, 医学部, 医員 (30526843)
• Project Period (FY): 2010 – 2012
• Keywords: 肺癌 / 一塩基多型 / SNP / SmartAmp 法 / 喫煙

Research Abstract

MDM2 is known as an oncoprotein that binds to p53 protein and inactivates the tumor suppressor activity of p53. Genetic polymorphisms in the MDM2 gene are suggested to be a tumor susceptibility marker and a prognostic factor for cancer. In this study, we have developed a new SNP detection method applied Smart Amplification Process (SmartAmp) method to detect the SNP c.309T>G in the MDM2 gene and conducted a large-scale case-controlled study. We found that there were no significant associations between MDM2 SNP309 genotypes and lung cancer risk. Subgroup analysis of smoking related lung cancer indicated that G/G genotype of SNP309 may be associated with lung cancer carcinogenesis.

Research Products

• [Journal Article] Rapid Detection of SNP (c.309T>G) in the MDM2 Gene by the Duplex SmartAmp Method (2013)
  • Author(s): Hanami, Yuki Kawai, Kengo Usui, Yasuko Okano, Seiichi Kakegawa, Hiroomi Ogawa, Yohei Miyamae, Yohei Miyagi, Haruhiko Nakayama, Toshihisa Ishikawa, Yoshihide Hayashizaki, Izumi Takeyoshi
  • Journal Title: PLOS ONE Volume: 8(4) Pages: e60151
  • DOI: doi:10.1371/journal.pone.0060151
  • Peer Reviewed
• [Presentation] 肺扁平上皮癌におけるMDM2遺伝子多型と喫煙との関連 (2012)
  • Author(s): 渥實潤、清水公裕ら
  • Organizer: 第53回日本肺癌学会総会
  • Place of Presentation: 岡山
  • Year and Date: 2012-11-09
• [Presentation] Smartamplification process(SmartAmp)法を応用した超高速MDM2遺伝子多型診断キットの開発 (2012)
  • Author(s): 榎田泰明、清水公裕ら
  • Organizer: 第112回日本外科学会定期学術集会
  • Place of Presentation: 幕張
  • Year and Date: 2012-04-14
• [Presentation] 女性肺腺癌におけるMDM2遺伝子多型と予後との関連についての検討 (2012)
  • Author(s): 渥實潤、清水公裕ら
  • Organizer: 第112回日本外科学会定期学術集会
  • Place of Presentation: 幕張
  • Year and Date: 2012-04-13
• [Presentation] ら肺腺癌におけるMDM2遺伝子多型と喫煙、EGFR遺伝子変異との関連についての検討 (2011)
  • Author(s): 懸川誠一、清水公裕
  • Organizer: 第52回日本肺癌学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2011-11-03
• [Presentation] MDM2SNP 309 is associated with cigarette smoking quantity in EGFR wild-type adenocarcinoma of the lung (2011)
  • Author(s): Jun Atsumi, Kimihiro Shimizu
  • Organizer: the 14th World Conference on Lung Cancer
  • Place of Presentation: Amsterdam, Netherlands
  • Year and Date: 2011-07-07